Abstract
Complementary DNA clones representing the 3' half, the 5' half, and the entire coding region of the human terminal deoxynucleotidyltransferase gene (TdT; DNA nucleotidylexotransferase, nucleosidetriphosphate: DNA deoxynucleotidylexotransferase, EC 2.7.7.31) were used to screen a panel of mouse X human somatic cell hybrid DNAs to determine the chromosomal location of the human TdT gene. The results of the Southern transfer analysis of hybrid DNAs indicate that the gene for TdT is located on human chromosome 10. The in situ hybridization technique was then used to further localize the gene for TdT to region q23-q25 of human chromosome 10.
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- Bollum F. J. Terminal deoxynucleotidyl transferase as a hematopoietic cell marker. Blood. 1979 Dec;54(6):1203–1215. [PubMed] [Google Scholar]
- Cannizzaro L. A., Emanuel B. S. An improved method for G-banding chromosomes after in situ hybridization. Cytogenet Cell Genet. 1984;38(4):308–309. doi: 10.1159/000132079. [DOI] [PubMed] [Google Scholar]
- Cooper C. S., Park M., Blair D. G., Tainsky M. A., Huebner K., Croce C. M., Vande Woude G. F. Molecular cloning of a new transforming gene from a chemically transformed human cell line. Nature. 1984 Sep 6;311(5981):29–33. doi: 10.1038/311029a0. [DOI] [PubMed] [Google Scholar]
- Croce C. M., Isobe M., Palumbo A., Puck J., Ming J., Tweardy D., Erikson J., Davis M., Rovera G. Gene for alpha-chain of human T-cell receptor: location on chromosome 14 region involved in T-cell neoplasms. Science. 1985 Mar 1;227(4690):1044–1047. doi: 10.1126/science.3919442. [DOI] [PubMed] [Google Scholar]
- Croce C. M., Nowell P. C. Molecular basis of human B cell neoplasia. Blood. 1985 Jan;65(1):1–7. [PubMed] [Google Scholar]
- Croce C. M., Shander M., Martinis J., Cicurel L., D'Ancona G. G., Dolby T. W., Koprowski H. Chromosomal location of the genes for human immunoglobulin heavy chains. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3416–3419. doi: 10.1073/pnas.76.7.3416. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dalla-Favera R., Bregni M., Erikson J., Patterson D., Gallo R. C., Croce C. M. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7824–7827. doi: 10.1073/pnas.79.24.7824. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dalla-Favera R., Franchini G., Martinotti S., Wong-Staal F., Gallo R. C., Croce C. M. Chromosomal assignment of the human homologues of feline sarcoma virus and avian myeloblastosis virus onc genes. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4714–4717. doi: 10.1073/pnas.79.15.4714. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Desiderio S. V., Yancopoulos G. D., Paskind M., Thomas E., Boss M. A., Landau N., Alt F. W., Baltimore D. Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells. Nature. 1984 Oct 25;311(5988):752–755. doi: 10.1038/311752a0. [DOI] [PubMed] [Google Scholar]
- Erikson J., Martinis J., Croce C. M. Assignment of the genes for human lambda immunoglobulin chains to chromosome 22. Nature. 1981 Nov 12;294(5837):173–175. doi: 10.1038/294173a0. [DOI] [PubMed] [Google Scholar]
- Green L., Van Antwerpen R., Stein J., Stein G., Tripputi P., Emanuel B., Selden J., Croce C. A major human histone gene cluster on the long arm of chromosome 1. Science. 1984 Nov 16;226(4676):838–840. doi: 10.1126/science.6494913. [DOI] [PubMed] [Google Scholar]
- Harper M. E., Saunders G. F. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. doi: 10.1007/BF00327364. [DOI] [PubMed] [Google Scholar]
- Hecht F., Morgan R., Hecht B. K., Smith S. D. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science. 1984 Dec 21;226(4681):1445–1447. doi: 10.1126/science.6438800. [DOI] [PubMed] [Google Scholar]
- Hedrick S. M., Nielsen E. A., Kavaler J., Cohen D. I., Davis M. M. Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins. Nature. 1984 Mar 8;308(5955):153–158. doi: 10.1038/308153a0. [DOI] [PubMed] [Google Scholar]
- Huebner K., Palumbo A. P., Isobe M., Kozak C. A., Monaco S., Rovera G., Croce C. M., Curtis P. J. The alpha-spectrin gene is on chromosome 1 in mouse and man. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3790–3793. doi: 10.1073/pnas.82.11.3790. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janossy G., Bollum F. J., Bradstock K. F., McMichael A., Rapson N., Greaves M. F. Terminal transferase-positive human bone marrow cells exhibit the antigenic phenotype of common acute lymphoblastic leukemia. J Immunol. 1979 Oct;123(4):1525–1529. [PubMed] [Google Scholar]
- Miyamoto K., Tomita N., Ishii A., Nonaka H., Kondo T., Tanaka T., Kitajima K. Chromosome abnormalities of leukemia cells in adult patients with T-cell leukemia. J Natl Cancer Inst. 1984 Aug;73(2):353–362. doi: 10.1093/jnci/73.2.353. [DOI] [PubMed] [Google Scholar]
- Peterson R. C., Cheung L. C., Mattaliano R. J., Chang L. M., Bollum F. J. Molecular cloning of human terminal deoxynucleotidyltransferase. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4363–4367. doi: 10.1073/pnas.81.14.4363. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sarin P. S., Gallo R. C. Terminal deoxynucleotidyltransferase in chronic myelogenous leukemia. J Biol Chem. 1974 Dec 25;249(24):8051–8053. [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Tsujimoto Y., Finger L. R., Yunis J., Nowell P. C., Croce C. M. Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation. Science. 1984 Nov 30;226(4678):1097–1099. doi: 10.1126/science.6093263. [DOI] [PubMed] [Google Scholar]
- Tsujimoto Y., Jaffe E., Cossman J., Gorham J., Nowell P. C., Croce C. M. Clustering of breakpoints on chromosome 11 in human B-cell neoplasms with the t(11;14) chromosome translocation. Nature. 1985 May 23;315(6017):340–343. doi: 10.1038/315340a0. [DOI] [PubMed] [Google Scholar]
- Tsujimoto Y., Yunis J., Onorato-Showe L., Erikson J., Nowell P. C., Croce C. M. Molecular cloning of the chromosomal breakpoint of B-cell lymphomas and leukemias with the t(11;14) chromosome translocation. Science. 1984 Jun 29;224(4656):1403–1406. doi: 10.1126/science.6610211. [DOI] [PubMed] [Google Scholar]