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. 1985 Oct;82(19):6427–6430. doi: 10.1073/pnas.82.19.6427

RNase T is responsible for the end-turnover of tRNA in Escherichia coli.

M P Deutscher, C W Marlor, R Zaniewski
PMCID: PMC390729  PMID: 2413440

Abstract

A mutant strain deficient in RNase T was isolated and used to study the role of this enzyme in Escherichia coli. Strains lacking as much as 70% of RNase T activity, alone or in combination with the absence of other RNases, display normal growth properties. However, in cca strains, which lack tRNA nucleotidyltransferase, RNase T-deficient derivatives accumulate lower levels of defective tRNA and grow at increased rates compared to their RNase T+ parents. Slow-growing cca strains revert to a faster-growing form that contains less defective tRNA but which is still cca. All of these strains have decreased levels of RNase T. These data indicate that RNase T is responsible for nucleotide removal during the tRNA end-turnover process and that the amount of defective tRNA in cells is determined by the relative levels of RNase T and tRNA nucleotidyltransferase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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