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. 1985 Dec;82(23):7904–7908. doi: 10.1073/pnas.82.23.7904

Exposure of actin thiols by the removal of tightly held calcium ions.

K Konno, M F Morales
PMCID: PMC390878  PMID: 3865205

Abstract

The removal of bound metal ions from G-actin uncovered two thiols, Cys-10 and Cys-257. The uncovering of these thiols requires a free calcium concentration lower than 10 nM. Therefore, participation of one or both thiols in Ca2+ binding is suggested. Actin labeled with N-(5-fluoresceinyl)maleimide in the absence of calcium moves as a doublet in NaDodSO4/PAGE. It is suggested that two conformers are induced by metal removal and labeling.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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