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. 1984 Sep;81(17):5532–5536. doi: 10.1073/pnas.81.17.5532

Expression of the Thy-1 glycoprotein gene by DNA-mediated gene transfer.

G A Evans, H A Ingraham, K Lewis, K Cunningham, T Seki, T Moriuchi, H C Chang, J Silver, R Hyman
PMCID: PMC391740  PMID: 6147849

Abstract

We isolated a gene encoding the Thy-1.2 glycoprotein from a recombinant library constructed from BALB/c mouse DNA. To evaluate the expression of this cloned gene in different genomic environments, we introduced it into cell lines derived from fibroblast, lymphoid, and neuronal tissues by DNA-mediated gene transfer. When integrated into the genome of mouse L cells, cell-surface Thy-1 can be detected with anti-Thy-1 monoclonal antibodies. These L-cell lines contain between two and four copies of the cloned Thy-1 gene stably integrated in the host genome. After subcloning into a plasmid vector containing the bacterial Eco-gpt gene as a selectable marker, the Thy-1 gene was introduced into the Thy-1-deficient mouse lymphoma AKR1 (Thy-1-d), and the rat neuronal cell line, B50. The resulting transformants also contain two to four copies of the cloned Thy-1 gene but express up to 50-fold more cell-surface Thy-1.2 than the L cell transformants. The expression of vastly differing amounts of cell-surface Thy-1 from similar numbers of genes suggests that the gene encoding this differentiation antigen is under tissue-specific regulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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