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. 1984 Sep;81(18):5714–5718. doi: 10.1073/pnas.81.18.5714

Restriction endonucleases can be used to study B-Z junctions in supercoiled DNA.

F Azorin, R Hahn, A Rich
PMCID: PMC391781  PMID: 6091104

Abstract

Plasmids containing (C-G)n inserts have been used to study the inhibition of cleavage by restriction endonucleases due to Z-DNA formation in negatively supercoiled plasmids. The enzyme BssHII, which recognizes G-C-G-C-G-C, is strongly inhibited when the insert forms Z-DNA. The BamHI recognition sequence (G-G-A-T-C-C) was placed in four different positions near the B-Z junction and the inhibition of BamHI cleavage was determined as a function of negative superhelical density. Formation of Z-DNA in the (C-G)n insert inhibited cleavage by BamHI when its recognition sequence was located immediately adjacent to the insert or four base pairs away from it. However, no inhibition was found when the BamHI recognition site was eight base pairs away. These experiments help to define the limits of the structural perturbation associated with the B-Z junction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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