Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Oct;81(19):6080–6084. doi: 10.1073/pnas.81.19.6080

Extension of bacteriophage lambda host range: selection, cloning, and characterization of a constitutive lambda receptor gene.

G E de Vries, C K Raymond, R A Ludwig
PMCID: PMC391863  PMID: 6091132

Abstract

A set of plasmids has been constructed that carry a constitutive lamB gene (LamBc phenotype) from Escherichia coli and that confer functional phage lambda receptors to bacteria other than E. coli. This E. coli LamBc strain has been selected to escape both maltose-inducible and glucose-repressible control. Constitutivity results from an IS-3 insertion, carrying a mobile promoter, proximal to lamB. The LamBc DNA has been cloned into both broad and narrow host-range plasmids, and the resulting pTROY plasmids have been transferred to diverse bacteria. Both Salmonella typhimurium/pTROY and Klebsiella pneumoniae/pTROY strains efficiently adsorb phage lambda; Pseudomonas aeruginosa/pTROY strains do not. Introduction of a functional E. coli LamB protein into foreign bacterial will allow these bacteria carrying pTROY plasmids to be infected by phage lambda recombinant DNA libraries, phage lambda::Tn insertion mutagenesis vectors, and in vivo lambda-packaged cosmids.

Full text

PDF
6080

Images in this article

6082Image
on p.6082
6083Image
on p.6083

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avery L., Kaiser D. In situ transposon replacement and isolation of a spontaneous tandem genetic duplication. Mol Gen Genet. 1983;191(1):99–109. doi: 10.1007/BF00330896. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bavoil P., Nikaido H. Physical interaction between the phage lambda receptor protein and the carrier-immobilized maltose-binding protein of Escherichia coli. J Biol Chem. 1981 Nov 25;256(22):11385–11388. [PubMed] [Google Scholar]
  4. Bedouelle H., Hofnung M. A DNA sequence containing the control regions of the malEFG and malK-lamB operons in Escherichia coli K12. Mol Gen Genet. 1982;185(1):82–87. doi: 10.1007/BF00333794. [DOI] [PubMed] [Google Scholar]
  5. Berg D. E., Egner C., Hirschel B. J., Howard J., Johnsrud L., Jorgensen R. A., Tlsty T. D. Insertion, excision, and inversion of Tn5. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):115–123. doi: 10.1101/sqb.1981.045.01.020. [DOI] [PubMed] [Google Scholar]
  6. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  7. Braun-Breton C., Hofnung M. Explanations accounting for transduction by bacteriophage lambda in maltose negative bacteriophage lambda resistant mutants of Escherichia coli K-12. Mol Gen Genet. 1978 Feb 16;159(2):143–149. doi: 10.1007/BF00270887. [DOI] [PubMed] [Google Scholar]
  8. Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976 Jul 5;104(3):541–555. doi: 10.1016/0022-2836(76)90119-4. [DOI] [PubMed] [Google Scholar]
  9. Chapon C. Role of the catabolite activator protein in the maltose regulon of Escherichia coli. J Bacteriol. 1982 May;150(2):722–729. doi: 10.1128/jb.150.2.722-729.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Charlier D., Piette J., Glansdorff N. IS3 can function as a mobile promoter in E. coli. Nucleic Acids Res. 1982 Oct 11;10(19):5935–5948. doi: 10.1093/nar/10.19.5935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Clement J. M., Perrin D., Hedgpeth J. Analysis of lambda receptor and beta-lactamase synthesis and export using cloned genes in a minicell system. Mol Gen Genet. 1982;185(2):302–310. doi: 10.1007/BF00330802. [DOI] [PubMed] [Google Scholar]
  12. Clément J. M., Hofnung M. Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12. Cell. 1981 Dec;27(3 Pt 2):507–514. doi: 10.1016/0092-8674(81)90392-5. [DOI] [PubMed] [Google Scholar]
  13. Collins J., Hohn B. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4242–4246. doi: 10.1073/pnas.75.9.4242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Colonna B., Hofnung M. rho Mutations restore lamB expression in E. coli K12 strains with an inactive malB region. Mol Gen Genet. 1981;184(3):479–483. doi: 10.1007/BF00352526. [DOI] [PubMed] [Google Scholar]
  15. Ditta G., Stanfield S., Corbin D., Helinski D. R. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347–7351. doi: 10.1073/pnas.77.12.7347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Dixon R., Kennedy C., Kondorosi A., Krishnapillai V., Merrick M. Complementation analysis of Klebsiella pneumoniae mutants defective in nitrogen fixation. Mol Gen Genet. 1977 Nov 29;157(2):189–198. doi: 10.1007/BF00267397. [DOI] [PubMed] [Google Scholar]
  17. Débarbouillé M., Shuman H. A., Silhavy T. J., Schwartz M. Dominant constitutive mutations in malT, the positive regulator gene of the maltose regulon in Escherichia coli. J Mol Biol. 1978 Sep 15;124(2):359–371. doi: 10.1016/0022-2836(78)90304-2. [DOI] [PubMed] [Google Scholar]
  18. Enomoto M., Stocker B. A. Transduction by phage P1kc in Salmonella typhimurium. Virology. 1974 Aug;60(2):503–514. doi: 10.1016/0042-6822(74)90344-4. [DOI] [PubMed] [Google Scholar]
  19. Ferenci T., Boos W. The role of the Escherichia coli lambda receptor in the transport of maltose and maltodextrins. J Supramol Struct. 1980;13(1):101–116. doi: 10.1002/jss.400130110. [DOI] [PubMed] [Google Scholar]
  20. Friedman A. M., Long S. R., Brown S. E., Buikema W. J., Ausubel F. M. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene. 1982 Jun;18(3):289–296. doi: 10.1016/0378-1119(82)90167-6. [DOI] [PubMed] [Google Scholar]
  21. Gabay J., Yasunaka K. Interaction of the lamB protein with the peptidoglycan layer in Escherichia coli K12. Eur J Biochem. 1980 Feb;104(1):13–18. doi: 10.1111/j.1432-1033.1980.tb04393.x. [DOI] [PubMed] [Google Scholar]
  22. Gilson E., Nikaido H., Hofnung M. Sequence of the malK gene in E.coli K12. Nucleic Acids Res. 1982 Nov 25;10(22):7449–7458. doi: 10.1093/nar/10.22.7449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Hall M. N., Silhavy T. J. Genetic analysis of the major outer membrane proteins of Escherichia coli. Annu Rev Genet. 1981;15:91–142. doi: 10.1146/annurev.ge.15.120181.000515. [DOI] [PubMed] [Google Scholar]
  24. Hu M., Deonier R. C. Comparison of IS1, IS2 and IS3 copy number in Escherichia coli strains K-12, B and C. Gene. 1981 Dec;16(1-3):161–170. doi: 10.1016/0378-1119(81)90072-x. [DOI] [PubMed] [Google Scholar]
  25. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Janssen D. B., Herst P. M., Joosten H. M., van der Drift C. Nitrogen control in Pseudomonas aeruginosa: a role for glutamine in the regulations of the synthesis of nadp-dependent glutamate dehydrogenase, urease and histidase. Arch Microbiol. 1981 Feb;128(4):398–402. doi: 10.1007/BF00405920. [DOI] [PubMed] [Google Scholar]
  27. Kennedy C. Linkage map of the nitrogen fixation (nif) genes in Klebsiella pneumoniae. Mol Gen Genet. 1977 Nov 29;157(2):199–204. doi: 10.1007/BF00267398. [DOI] [PubMed] [Google Scholar]
  28. Miller J. H., Ganem D., Lu P., Schmitz A. Genetic studies of the lac repressor. I. Correlation of mutational sites with specific amino acid residues: construction of a colinear gene-protein map. J Mol Biol. 1977 Jan 15;109(2):275–298. doi: 10.1016/s0022-2836(77)80034-x. [DOI] [PubMed] [Google Scholar]
  29. Raibaud O., Clément J. M., Hofnung M. Structure of the malB region in Escherichia coli K12. III. Correlation of the genetic map with the restriction map. Mol Gen Genet. 1979 Jul 24;174(3):261–267. doi: 10.1007/BF00267798. [DOI] [PubMed] [Google Scholar]
  30. Raibaud O., Roa M., Braun-Breton C., Schwartz M. Structure of the malB region in Escherichia coli K12. I. Genetic map of the malK-lamB operon. Mol Gen Genet. 1979 Jul 24;174(3):241–248. doi: 10.1007/BF00267796. [DOI] [PubMed] [Google Scholar]
  31. Randall-Hazelbauer L., Schwartz M. Isolation of the bacteriophage lambda receptor from Escherichia coli. J Bacteriol. 1973 Dec;116(3):1436–1446. doi: 10.1128/jb.116.3.1436-1446.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Roa M. Interaction of bacteriophage K10 with its receptor, the lamB protein of Escherichia coli. J Bacteriol. 1979 Nov;140(2):680–686. doi: 10.1128/jb.140.2.680-686.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Schwartz M., Le Minor L. Occurrence of the bacteriophage lambda receptor in some enterobacteriaceae. J Virol. 1975 Apr;15(4):679–685. doi: 10.1128/jvi.15.4.679-685.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Schwartz M. Reversible interaction between coliphage lambda and its receptor protein. J Mol Biol. 1975 Nov 25;99(1):185–201. doi: 10.1016/s0022-2836(75)80167-7. [DOI] [PubMed] [Google Scholar]
  35. Schwartz M. The adsorption of coliphage lambda to its host: effect of variations in the surface density of receptor and in phage-receptor affinity. J Mol Biol. 1976 May 25;103(3):521–536. doi: 10.1016/0022-2836(76)90215-1. [DOI] [PubMed] [Google Scholar]
  36. Silhavy T. J., Benson S. A., Emr S. D. Mechanisms of protein localization. Microbiol Rev. 1983 Sep;47(3):313–344. doi: 10.1128/mr.47.3.313-344.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Streicher S. L., Shanmugam K. T., Ausubel F., Morandi C., Goldberg R. B. Regulation of nitrogen fixation in Klebsiella pneumoniae: evidence for a role of glutamine synthetase as a regulator of nitrogenase synthesis. J Bacteriol. 1974 Nov;120(2):815–821. doi: 10.1128/jb.120.2.815-821.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Umene K., Shimada K., Takagi Y. Packaging of ColE1 DNA having a lambda phage cohesive end site. Mol Gen Genet. 1978 Feb 7;159(1):39–45. doi: 10.1007/BF00401746. [DOI] [PubMed] [Google Scholar]
  39. Wandersman C., Schwartz M., Ferenci T. Escherichia coli mutants impaired in maltodextrin transport. J Bacteriol. 1979 Oct;140(1):1–13. doi: 10.1128/jb.140.1.1-13.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Wandersman C., Schwartz M. Protein Ia and the lamB protein can replace each other in the constitution of an active receptor for the same coliphage. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5636–5639. doi: 10.1073/pnas.75.11.5636. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES