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. 1984 Nov;81(21):6681–6685. doi: 10.1073/pnas.81.21.6681

Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies.

A H Ross, P Grob, M Bothwell, D E Elder, C S Ernst, N Marano, B F Ghrist, C C Slemp, M Herlyn, B Atkinson, et al.
PMCID: PMC391994  PMID: 6093111

Abstract

The nerve growth factor (NGF) receptor was characterized by using a new series of anti-receptor monoclonal antibodies (MAbs). These MAbs (i) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor, (ii) inhibited the binding of 125I-labeled NGF to its receptor, and (iii) immunoprecipitated both metabolically labeled and 125I-labeled NGF affinity-labeled receptor. These experiments defined the receptor as a 75-kDa cell-surface protein. The NGF receptor was visualized by immunoperoxidase staining in tissue sections of human nevi, melanomas, neurofibromas, a pheochromocytoma, and peripheral nerves. Uniform staining of the cytoplasm suggests that, in addition to cell-surface NGF receptors, there is a population of intracellular receptors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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