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. 1984 Nov;81(21):6691–6695. doi: 10.1073/pnas.81.21.6691

Heat-labile alkaline phosphatase from Antarctic bacteria: Rapid 5′ end-labeling of nucleic acids

Hiromi Kobori *,†, Cornelius W Sullivan ‡, Hiroaki Shizuya *,§
PMCID: PMC391996  PMID: 16593525

Abstract

A heat-labile alkaline phosphatase has been purified to near homogeneity from HK47, a bacterial strain isolated from Antarctic seawater. The active form of the enzyme has a molecular weight of 68,000 and is uniquely monomeric. The optimal temperature for the enzymatic activity is 25°C. Complete and irreversible thermal inactivation of the enzyme occurs in 10 min at 55°C. By using this heat-labile enzyme for dephosphorylation followed by a 10-min heat treatment, rapid end-labeling of nucleic acids by T4 polynucleotide kinase has been achieved.

Keywords: radioactive labeling, polynucleotide kinase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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