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. 1984 Dec;81(24):7917–7921. doi: 10.1073/pnas.81.24.7917

Human interleukin 2 can promote the growth and differentiation of single hapten-specific B cells in the presence of specific antigen.

B L Pike, A Raubitschek, G J Nossal
PMCID: PMC392264  PMID: 6334854

Abstract

B lymphocytes specifically reactive to the hapten fluorescein (FLU) were prepared from normal adult murine spleen by the hapten-gelatin affinity procedure. They were placed in 10 microliter of microcultures singly or in small numbers in the absence of any feeder, filler, or accessory cell. The "T cell-independent" antigen FLU-conjugated polymerized flagellin (FLU-POL) or a selected batch of FLU-conjugated Ficoll were used, and these stimulated division and differentiation only in the concomitant presence of lymphokines acting as B-cell growth and differentiation factors (BGDF). It was found that human interleukin 2 (IL-2), prepared by recombinant DNA technology (r-IL-2), was an effective, albeit rather weak, BGDF in this system. When an IL-2-free source of BGDF was used with the antigenic stimulus, addition of r-IL-2 did not augment the response, nor did removal of IL-2 from the crude lymphokine mixture diminish the BGDF activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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