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. 1978 Apr;75(4):1844–1848. doi: 10.1073/pnas.75.4.1844

Growth of embryonal carcinoma cells in serum-free medium.

A Rizzino, G Sato
PMCID: PMC392437  PMID: 77018

Abstract

Two mouse embryonal carcinoma cell lines, PCC.4 aza-1 and F9, have been grown in serum-free F-12 medium supplemented with Pedersen fetuin, insulin, transferrin, and 2-mercaptoethanol. This medium supports long-term growth of both cell lines. When these cells are transferred from medium containing serum to this serum-free medium, growth continues without any detectable lag. PCC.4 aza-1 grown in this medium for over 20 generations retains the capacity to differentiate in vivo. This medium appears to be a general serum-free medium for the growth of embryonal carcinoma cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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