Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 May;75(5):2421–2423. doi: 10.1073/pnas.75.5.2421

Restricted classes of immunoglobulin produced by a lymphoid cell line from a patient with agammaglobulinemia.

J Schwaber, H Lazarus, F S Rosen
PMCID: PMC392565  PMID: 209464

Abstract

Restricted expression of immunoglobulin by a long-term B lymphoid cell line derived from a patient with X-linked agammaglobulinemia is reproted. The patient had peripheral blood B lymphocytes with surface IgD and IgM. Culture of the B cells in vitro with mitogens did not stimulate immunoglobulin secretion, although pokeweed mitogen stimulation resulted in the development of cytoplasmic IgD and IgM. The lymphoid cell line established from these B lymphocytes primarily bore surface IgD, with a small population of cells also bearing surface IgM. These cells also had cytoplasmic immunoglobulin, primarily IgD. The cell line did not have a cytoplasmic pool of immunoglobulin for export and did not secrete immunoglobulin. This B lymphoid cell line is an in vitro analogue of the arrest in differentiation of the patient's B lymphocytes. The IgD-bearing phenotype may represent a normal transitional stage in the differentiation of B lymphocytes.

Full text

PDF
2422

Images in this article

2422Image
on p.2422

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ault K. A., Unanue E. R. Comparison of the two types of immunoglobulin-bearing lymphocytes in human blood with regard to capping and motility. Clin Immunol Immunopathol. 1977 May;7(3):394–404. doi: 10.1016/0090-1229(77)90074-5. [DOI] [PubMed] [Google Scholar]
  2. Cooper M. D., Kearney J. F., Lawton A. R., Abney E. R., Parkhouse R. M., Preud'homme J. L., Seligmann M. Generation of immunoglobulin class diversity in b cells: a discussion with emphasis on idg development. Ann Immunol (Paris) 1976 Jun-Jul;127(3-4):573–581. [PubMed] [Google Scholar]
  3. Dickler H. B., Kunkel H. G. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. doi: 10.1084/jem.136.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Geha R. S., Rosen F. S., Merler E. Identification and characterization of subpopulations of lymphocytes in human peripheral blood after fractionation on discontinuous gradients of albumin. The cellular defect in X-linked agammaglobulinemia. J Clin Invest. 1973 Jul;52(7):1726–1734. doi: 10.1172/JCI107354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Greaves M., Janossy G. Elicitation of selective T and B lymphocyte responses by cell surface binding ligands. Transplant Rev. 1972;11:87–130. doi: 10.1111/j.1600-065x.1972.tb00047.x. [DOI] [PubMed] [Google Scholar]
  6. Kaplan J., Shope T. C., Peterson W. D., Jr Epstein-barr virus-negative human malignant T-cell lines. J Exp Med. 1974 May 1;139(5):1070–1076. doi: 10.1084/jem.139.5.1070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Litwin S. D., Hütteroth T. H., Lin P. K., Kennard J., Cleve H. Immunoglobulin expression of cells from human lymphoblastoid lines. II. Interrelationship among surface, cellular, and secreted immunoglobulins. J Immunol. 1974 Aug;113(2):668–672. [PubMed] [Google Scholar]
  8. Nilsson K., Pontén J. Classification and biological nature of established human hematopoietic cell lines. Int J Cancer. 1975 Feb 15;15(2):321–341. doi: 10.1002/ijc.2910150217. [DOI] [PubMed] [Google Scholar]
  9. Robinson J. Assay for Epstein-Barr virus based on stimulation of DNA synthesis in mixed leukocytes from human umbilical cord blood. J Virol. 1975 May;15(5):1065–1072. doi: 10.1128/jvi.15.5.1065-1072.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rowe D. S., Hug K., Faulk W. P., McCormick J. N., Gerber H. IgD on the surface of peripheral blood lymphocytes of the human newborn. Nat New Biol. 1973 Apr 4;242(118):155–157. doi: 10.1038/newbio242155a0. [DOI] [PubMed] [Google Scholar]
  11. Rowe D. S., Hug K., Forni L., Pernis B. Immunoglobulin D as a lymphocyte receptor. J Exp Med. 1973 Oct 1;138(4):965–972. doi: 10.1084/jem.138.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schwaber J. Immunoglobulin production by a human-mouse somatic cell hybrid. Exp Cell Res. 1975 Jul;93(2):343–354. doi: 10.1016/0014-4827(75)90459-0. [DOI] [PubMed] [Google Scholar]
  13. Vitetta E. S., Uhr J. W. Immunoglobulin-receptors revisited. Science. 1975 Sep 19;189(4207):964–969. doi: 10.1126/science.1083069. [DOI] [PubMed] [Google Scholar]
  14. van Boxel J. A., Buell D. N. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature. 1974 Oct 4;251(5474):443–444. doi: 10.1038/251443a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES