Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 Aug;75(8):3673–3677. doi: 10.1073/pnas.75.8.3673

Low molecular weight RNA species encoded by a multiple drug resistance plasmid.

M De Wilde, J E Davies, F J Schmidt
PMCID: PMC392848  PMID: 358192

Abstract

Multiple drug resistance plasmid NR1 is shown to code for at least 10 low molecular weight RNAs. These species, ranging in size from 60 to 120 nucleotides, have been purified from minicells by two-dimensional gel electrophoresis and characterized by RNase T1 fingerprinting. Hybridization of purified RNAs to restriction endonuclease digests of NR1 DNA indicates that most are derived from the resistance transfer factor region of the plasmid genome. One RNA was found to be coded by the transposable tetracycline resistance element Tn10, and several are associated with DNA fragments that contain origins of replication.

Full text

PDF
3676

Images in this article

3674Image
on p.3674
3674Image
on p.3674
3674Image
on p.3674
3674Image
on p.3674
3675Image
on p.3675
3675Image
on p.3675
3675Image
on p.3675
3675Image
on p.3675
3675Image
on p.3675
3675Image
on p.3675
3676Image
on p.3676

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bertrand K., Korn L., Lee F., Platt T., Squires C. L., Squires C., Yanofsky C. New features of the regulation of the tryptophan operon. Science. 1975 Jul 4;189(4196):22–26. doi: 10.1126/science.1094538. [DOI] [PubMed] [Google Scholar]
  3. Frazer A. C., Curtiss R., 3rd Production, properties and utility of bacterial minicells. Curr Top Microbiol Immunol. 1975;69:1–84. doi: 10.1007/978-3-642-50112-8_1. [DOI] [PubMed] [Google Scholar]
  4. Guthrie C., McClain W. H. Conditionally lethal mutants of bacteriophage T4 defective in production of a transfer RNA. J Mol Biol. 1973 Dec 5;81(2):137–155. doi: 10.1016/0022-2836(73)90185-x. [DOI] [PubMed] [Google Scholar]
  5. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  6. Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. J Biol Chem. 1973 Jul 25;248(14):5024–5032. [PubMed] [Google Scholar]
  7. Landy A., Abelson J., Goodman H. M., Smith J. D. Specific hybridization of tyrosine transfer ribonucleic acids with DNA from a transducing bacteriophage phi-80 carrying the amber suppressor gene su 3. J Mol Biol. 1967 Nov 14;29(3):457–471. doi: 10.1016/0022-2836(67)90112-x. [DOI] [PubMed] [Google Scholar]
  8. Lennarz W. J., Nesbitt J. A., 3rd, Reiss J. The participation of sRNA in the enzymatic synthesis of O-L-lysyl phosphatidylgylcerol in Staphylococcus aureus. Proc Natl Acad Sci U S A. 1966 Apr;55(4):934–941. doi: 10.1073/pnas.55.4.934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McClain W. H., Guthrie C., Barrell B. G. Eight transfer RNAs induced by infection of Escherichia coli with bacteriophage T4. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3703–3707. doi: 10.1073/pnas.69.12.3703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  11. Morgan E. A., Ikemura T., Nomura M. Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2710–2714. doi: 10.1073/pnas.74.7.2710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NAKAYA R., NAKAMURA A., MURATA Y. Resistance transfer agents in Shigella. Biochem Biophys Res Commun. 1960 Dec;3:654–659. doi: 10.1016/0006-291x(60)90081-4. [DOI] [PubMed] [Google Scholar]
  13. Roberts R. J. Structures of two glycyl-tRNAs from Staphylococcus epidermidis. Nat New Biol. 1972 May 10;237(71):44–45. doi: 10.1038/newbio237044a0. [DOI] [PubMed] [Google Scholar]
  14. Roozen K. J., Fenwick R. G., Jr, Curtiss R., 3rd Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12. J Bacteriol. 1971 Jul;107(1):21–33. doi: 10.1128/jb.107.1.21-33.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Saneyoshi M., Oashi Z., Harada F., Nishimura S. Isolation and characterization of 2-methyladenosine from Escherichia coli tRNA Glu 2 , tRNA Asp 1 , tRNA His 1 and tRNA Arg . Biochim Biophys Acta. 1972 Feb 23;262(1):1–10. [PubMed] [Google Scholar]
  16. Sharp P. A., Cohen S. N., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol. 1973 Apr 5;75(2):235–255. doi: 10.1016/0022-2836(73)90018-1. [DOI] [PubMed] [Google Scholar]
  17. Skurray R. A., Nagaishi H., Clark A. J. Molecular cloning of DNA from F sex factor of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1976 Jan;73(1):64–68. doi: 10.1073/pnas.73.1.64. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Sugino Y., Tomizawa J., Kakefuda T. Location of non-DNA components of closed circular colicin E1 plasmid DNA. Nature. 1975 Feb 20;253(5493):652–654. doi: 10.1038/253652a0. [DOI] [PubMed] [Google Scholar]
  20. Tanak N., Cramer J. H., Rownd R. H. EcoRI restriction endonuclease map of the composite R plasmid NR1. J Bacteriol. 1976 Jul;127(1):619–636. doi: 10.1128/jb.127.1.619-636.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Williams H., Boyer H. W., Helsinki D. R. Size and base composition of RNA in supercoiled plasmid DNA. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3744–3748. doi: 10.1073/pnas.70.12.3744. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES