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. 1978 Dec;75(12):5765–5769. doi: 10.1073/pnas.75.12.5765

Chemical synthesis of genes for human insulin.

R Crea, A Kraszewski, T Hirose, K Itakura
PMCID: PMC393054  PMID: 282602

Abstract

A rapid chemical procedure has been developed and used for the synthesis of 29 oligodeoxyribonucleotides to build synthetic genes for human insulin. The gene for insulin B chain, 104 base pairs, and the one for A chain, 77 base pairs, were designed from the amino acid sequence of human polypeptides. They bear single-stranded cohesive termini for the EcoRI and BamHI restriction endonucleases and are designed to be inserted separately into a pBR322 plasmid. The synthetic fragments, deca- to pentadecanucleotides, were synthesized by a block phosphotriester method with trinucleotides as building blocks. Final purification was by high-performance liquid chromatography. All 29 oligonucleotides were pure and had the correct sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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