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. 1983 Mar;80(5):1421–1425. doi: 10.1073/pnas.80.5.1421

Isolation and characterization of a temperature-sensitive mutant of avian myeloblastosis virus.

M G Moscovici, C Moscovici
PMCID: PMC393609  PMID: 6298790

Abstract

A temperature-sensitive (ts) mutant, GA 907/7, was isolated after mutagen treatment of avian myeloblastosis virus. When bone marrow cells or secondary yolk sac macrophages were infected with GA 907/7, the expression of transformation was greatly reduced at 41 degrees C. The results of temperature-shift experiments suggest that in GA 907/7 the putative v-myb gene product is functional only at 35.5 degrees C. Moreover, when ts-induced transformed cells were shifted to 41 degrees C, a partial morphological conversion to macrophage-like cells was obtained, while the majority of the cells underwent senescence and lysis. No leukemia was obtained when GA 907/7 was injected in 1-day-old chickens. Finally, a continuous cell line releasing genetically stable mutant virus was obtained after transformation of secondary yolk sac cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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