Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Mar;80(5):1445–1448. doi: 10.1073/pnas.80.5.1445

Identification of a second class I antigen controlled by the K end of the H-2 complex and its selective cellular expression.

M Tryphonas, D P King, P P Jones
PMCID: PMC393614  PMID: 6572399

Abstract

Immunoprecipitates obtained from [35S]methionine-labeled spleen cells by using monoclonal antibodies specific for H-2Kd and H-2Dd have been separated by two-dimensional polyacrylamide gel electrophoresis. Analysis of these gel patterns revealed the presence of an additional product of the K end of the H-2d complex, designated here as H-2K'. To determine whether H-2K' is a unique protein or a differentially glycosylated form of the previously characterized H-2Kd histocompatibility antigen, nonglycosylated molecules labeled in the presence of tunicamycin were examined. The results showed that both H-2K and H-2K' have distinct nonglycosylated polypeptide precursor forms. The approximate molecular weight differences between the fully glycosylated and nonglycosylated molecules also indicated the presence of three oligosaccharide side chains on H-2K', as is the case with H-2Kd, whereas H-2Dd has only two oligosaccharide units. The cellular expression of H-2K' was also investigated. Comparison of H-2 antigens immunoprecipitated from normal spleen cells and from thioglycollate-induced adherent peritoneal exudate cells cultured in the presence or absence of supernatant fluids from concanavalin A-stimulated spleen cells revealed that H-2K' was not expressed on the adherent peritoneal cells. This indicates that H-2K' is expressed in a tissue-specific manner, unlike the classical histocompatibility antigens H-2K and H-2D.

Full text

PDF
1447

Images in this article

1446Image
on p.1446
1447Image
on p.1447
1448Image
on p.1448

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cosman D., Khoury G., Jay G. Three classes of mouse H-2 messenger RNA distinguished by analysis of cDNA clones. Nature. 1982 Jan 7;295(5844):73–76. doi: 10.1038/295073a0. [DOI] [PubMed] [Google Scholar]
  2. Doherty P. C., Zinkernagel R. M. H-2 compatibility is required for T-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus. J Exp Med. 1975 Feb 1;141(2):502–507. doi: 10.1084/jem.141.2.502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Démant P., Iványi D. Further molecular complexities of H-2 K- and D-region antigens. Nature. 1981 Mar 12;290(5802):146–149. doi: 10.1038/290146a0. [DOI] [PubMed] [Google Scholar]
  4. Démant P., Iványi D., Oudshoorn-Snoek M., Calafat J., Roos M. H. Molecular heterogeneity of H-2 antigens. Immunol Rev. 1981;60:5–22. doi: 10.1111/j.1600-065x.1981.tb00360.x. [DOI] [PubMed] [Google Scholar]
  5. Démant P., Roos M. H. Molecular heterogeneity of D-end products detected by anti-H-2.28 sera. I. A. molecule similar to Qa-2, detected in the BALB/cBy but not in the BALB/c-H-2dm2 mutant. Immunogenetics. 1982;15(5):461–466. doi: 10.1007/BF00345905. [DOI] [PubMed] [Google Scholar]
  6. Hansen T. H., Ozato K., Melino M. R., Coligan J. E., Kindt T. J., Jandinski J. J., Sachs D. H. Immunochemical evidence in two haplotypes for at least three D region-encoded molecules, D, L, and R. J Immunol. 1981 May;126(5):1713–1716. [PubMed] [Google Scholar]
  7. Iványi D., Démant P. Molecular heterogeneity of D-end products detected by anti-H-2.28 sera. II. B10.D2(M504) (H-2dm1) mutant fails to express one of the two H-2.4-, 28 + Dd region molecules. Immunogenetics. 1982;15(5):467–476. doi: 10.1007/BF00345906. [DOI] [PubMed] [Google Scholar]
  8. Iványi D., Démant P. Serological characterization of previously unknown H-2 molecules identified in the products of the Kd and Dk region. Immunogenetics. 1981;12(3-4):397–408. doi: 10.1007/BF01561679. [DOI] [PubMed] [Google Scholar]
  9. Jones P. P. Aberrant Ae (E beta) Ia polypeptide chain in H-2g2 haplotype mice. Possible result of an intragenic recombination or mutation. J Exp Med. 1980 Nov 1;152(5):1453–1458. doi: 10.1084/jem.152.5.1453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones P. P. Analysis of H-2 and Ia molecules by two-dimensional gel electrophoresis. J Exp Med. 1977 Nov 1;146(5):1261–1279. doi: 10.1084/jem.146.5.1261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kimball E. S., Maloy W. L., Coligan J. E. Evidence for three carbohydrate prosthetic groups on mouse histocompatibility antigens H-2Kd and H-2Db. Mol Immunol. 1981 Jul;18(7):677–680. doi: 10.1016/0161-5890(81)90039-0. [DOI] [PubMed] [Google Scholar]
  12. Klein J., Shreffler D. C. The H-2 model for the major histocompatibility systems. Transplant Rev. 1971;6:3–29. doi: 10.1111/j.1600-065x.1971.tb00457.x. [DOI] [PubMed] [Google Scholar]
  13. Krakauer T., Hansen T. H., Camerini-Otero R. D., Sachs D. H. Analysis of the heterogeneity of the mouse H-2K, D, and L gene products. J Immunol. 1980 May;124(5):2149–2156. [PubMed] [Google Scholar]
  14. Lehle L., Tanner W. The specific site of tunicamycin inhibition in the formation of dolichol-bound N-acetylglucosamine derivatives. FEBS Lett. 1976 Nov 15;72(1):167–170. doi: 10.1016/0014-5793(76)80922-2. [DOI] [PubMed] [Google Scholar]
  15. McNicholas J. M., King D. P., Jones P. P. Biosynthesis and expression of Ia and H-2 antigens on a macrophage cell line are stimulated by products of activated spleen cells. J Immunol. 1983 Jan;130(1):449–456. [PubMed] [Google Scholar]
  16. Moosic J. P., Sung E., Nilson A., Jones P. P., McKean D. J. The selective solubilization of different murine splenocyte membrane fractions with lubrol WX and triton X-100 distinguishes two forms of Ia antigens. A cell surface (alpha, beta) and an intracellular (alpha, Ii, beta). J Biol Chem. 1982 Aug 25;257(16):9684–9691. [PubMed] [Google Scholar]
  17. Nairn R., Nathenson S. G., Coligan J. E. Isolation, characterization and amino acid sequence studies of the cyanogen bromide fragments of the H-2Dd glycoprotein. Eur J Immunol. 1980 Jul;10(7):495–503. doi: 10.1002/eji.1830100703. [DOI] [PubMed] [Google Scholar]
  18. Oi V. T., Jones P. P., Goding J. W., Herzenberg L. A., Herzenberg L. A. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978;81:115–120. doi: 10.1007/978-3-642-67448-8_18. [DOI] [PubMed] [Google Scholar]
  19. Ozato K., Mayer N. M., Sachs D. H. Monoclonal antibodies to mouse major histocompatibility complex antigens. Transplantation. 1982 Sep;34(3):113–120. doi: 10.1097/00007890-198209000-00001. [DOI] [PubMed] [Google Scholar]
  20. Ozato K., Sachs D. H. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981 Jan;126(1):317–321. [PubMed] [Google Scholar]
  21. Reyes A. A., Schöld M., Itakura K., Wallace R. B. Isolation of a cDNA clone for the murine transplantation antigen H-2Kb. Proc Natl Acad Sci U S A. 1982 May;79(10):3270–3274. doi: 10.1073/pnas.79.10.3270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Steinmetz M., Moore K. W., Frelinger J. G., Sher B. T., Shen F. W., Boyse E. A., Hood L. A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell. 1981 Sep;25(3):683–692. doi: 10.1016/0092-8674(81)90175-6. [DOI] [PubMed] [Google Scholar]
  23. Steinmetz M., Winoto A., Minard K., Hood L. Clusters of genes encoding mouse transplantation antigens. Cell. 1982 Mar;28(3):489–498. doi: 10.1016/0092-8674(82)90203-3. [DOI] [PubMed] [Google Scholar]
  24. Stern A. C., Erb P., Gisler R. H. Ia-bearing bone marrow-cultured macrophages induce antigen-specific helper T cells for antibody synthesis. J Immunol. 1979 Aug;123(2):612–615. [PubMed] [Google Scholar]
  25. Sung E., Jones P. P. The invariant chain of murine Ia antigens: its glycosylation, abundance and subcellular localization. Mol Immunol. 1981 Oct;18(10):899–913. doi: 10.1016/0161-5890(81)90013-4. [DOI] [PubMed] [Google Scholar]
  26. Tkacz J. S., Lampen O. Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes. Biochem Biophys Res Commun. 1975 Jul 8;65(1):248–257. doi: 10.1016/s0006-291x(75)80086-6. [DOI] [PubMed] [Google Scholar]
  27. Xin J. H., Kvist S., Dobberstein B. Identification of an H-2Kd gene using a specific cDNA probe. EMBO J. 1982;1(4):467–471. doi: 10.1002/j.1460-2075.1982.tb01192.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES