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. 1983 May;80(9):2666–2670. doi: 10.1073/pnas.80.9.2666

Escherichia coli extract-catalyzed recombination in switch regions of mouse immunoglobulin genes.

T Kataoka, S Takeda, T Honjo
PMCID: PMC393888  PMID: 6221341

Abstract

We have shown that Escherichia coli extracts catalyze recombination between mouse immunoglobulin mu and alpha genes inserted separately in lambda phage vectors carrying different genetic markers. Most of the recombination sites in the inserts are located in the switch regions of the heavy chain genes, as previously found in the expressed genes of myeloma cells. The recombination took place at relatively high frequency (10(-4)). The recombinational system in E. coli or lambda phage seems to prefer short nucleotide sequences similar to those used in the class switch recombination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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