Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Jun;80(12):3807–3811. doi: 10.1073/pnas.80.12.3807

Analysis of cell surface proteins delineates a differentiation pathway linking endocrine and nonendocrine human lung cancers.

G Goodwin, J H Shaper, M D Abeloff, G Mendelsohn, S B Baylin
PMCID: PMC394141  PMID: 6304739

Abstract

We have previously determined that the cell surface protein phenotype distinguishes human small cell lung carcinoma (SCC), a neoplasm with endocrine properties, from non-SCC in culture. We now demonstrate that cloned cell cultures of human large cell undifferentiated lung carcinoma, established directly from a patient with mixed SCC and non-SCC, simultaneously express surface proteins characteristic of SCC and non-SCC lung cancer cells. Hence, SCC and a form of large cell carcinoma appear linked through a continuum of differentiation events that: (i) may explain clinically important transitions that occur between the major types of human lung cancer and (ii) provide evidence for a common cellular origin of endocrine and nonendocrine cells in the bronchial mucosa.

Full text

PDF
3809

Images in this article

3808Image
on p.3808
3808Image
on p.3808
3808Image
on p.3808
3808Image
on p.3808
3808Image
on p.3808
3808Image
on p.3808
3809Image
on p.3809

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abeloff M. D., Eggleston J. C., Mendelsohn G., Ettinger D. S., Baylin S. B. Changes in morphologic and biochemical characteristics of small cell carcinoma of the lung. A clinicopathologic study. Am J Med. 1979 May;66(5):757–764. doi: 10.1016/0002-9343(79)91113-6. [DOI] [PubMed] [Google Scholar]
  2. Aisenberg A. C. Current concepts in immunology: Cell-surface markers in lymphoproliferative disease. N Engl J Med. 1981 Feb 5;304(6):331–336. doi: 10.1056/NEJM198102053040606. [DOI] [PubMed] [Google Scholar]
  3. Baylin S. B., Abeloff M. D., Goodwin G., Carney D. N., Gazdar A. F. Activities of L-dopa decarboxylase and diamine oxidase (histaminase) in human lung cancers and decarboxylase as a marker for small (oat) cell cancer in cell culture. Cancer Res. 1980 Jun;40(6):1990–1994. [PubMed] [Google Scholar]
  4. Baylin S. B., Gazdar A. F., Minna J. D., Bernal S. D., Shaper J. H. A unique cell-surface protein phenotype distinguishes human small-cell from non-small-cell lung cancer. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4650–4654. doi: 10.1073/pnas.79.15.4650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bensch K. G., Corrin B., Pariente R., Spencer H. Oat-cell carcinoma of the lung. Its origin and relationship to bronchial carcinoid. Cancer. 1968 Dec;22(6):1163–1172. doi: 10.1002/1097-0142(196811)22:6<1163::aid-cncr2820220612>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  6. Berger C. L., Goodwin G., Mendelsohn G., Eggleston J. C., Abeloff M. D., Aisner S., Baylin S. B. Endocrine-related biochemistry in the spectrum of human lung carcinoma. J Clin Endocrinol Metab. 1981 Aug;53(2):422–429. doi: 10.1210/jcem-53-2-422. [DOI] [PubMed] [Google Scholar]
  7. Brereton H. D., Mathews M. M., Costa J., Kent C. H., Johnson R. E. Mixed anaplastic small-cell and squamous-cell carcinoma of the lung. Ann Intern Med. 1978 Jun;88(6):805–806. doi: 10.7326/0003-4819-88-6-805. [DOI] [PubMed] [Google Scholar]
  8. Bunn P. A., Jr, Cohen M. H., Ihde D. C., Fossieck B. E., Jr, Matthews M. J., Minna J. D. Advances in small cell bronchogenic carcinoma. Cancer Treat Rep. 1977 May-Jun;61(3):333–342. [PubMed] [Google Scholar]
  9. Cantor H., Boyse E. A. Lymphocytes as models for the study of mammalian cellular differentiation. Immunol Rev. 1977 Jan;33:105–124. doi: 10.1111/j.1600-065x.1977.tb00364.x. [DOI] [PubMed] [Google Scholar]
  10. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  11. Gazdar A. F., Carney D. N., Russell E. K., Sims H. L., Baylin S. B., Bunn P. A., Jr, Guccion J. G., Minna J. D. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 1980 Oct;40(10):3502–3507. [PubMed] [Google Scholar]
  12. Goodwin G., Baylin S. B. Relationships between neuroendocrine differentiation and sensitivity to gamma-radiation in culture line OH-1 of human small cell lung carcinoma. Cancer Res. 1982 Apr;42(4):1361–1367. [PubMed] [Google Scholar]
  13. Hattori S., Matsuda M., Tateishi R., Nishihara H., Horai T. Oat-cell carcinoma of the lung. Clinical and morphological studies in relation to its histogenesis. Cancer. 1972 Oct;30(4):1014–1024. doi: 10.1002/1097-0142(197210)30:4<1014::aid-cncr2820300424>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
  14. Hsu S. H., Schacter B. Z., Delaney N. L., Miller T. B., McKusick V. A., Kennett R. H., Bodmer J. G., Young D., Bodmer W. F. Genetic characteristics of the HeLa cell. Science. 1976 Jan 30;191(4225):392–394. doi: 10.1126/science.1246620. [DOI] [PubMed] [Google Scholar]
  15. Lane M. A., Sainten A., Cooper G. M. Stage-specific transforming genes of human and mouse B- and T-lymphocyte neoplasms. Cell. 1982 Apr;28(4):873–880. doi: 10.1016/0092-8674(82)90066-6. [DOI] [PubMed] [Google Scholar]
  16. Marangos P. J., Gazdar A. F., Carney D. N. Neuron specific enolase in human small cell carcinoma cultures. Cancer Lett. 1982 Jan;15(1):67–71. doi: 10.1016/0304-3835(82)90077-5. [DOI] [PubMed] [Google Scholar]
  17. Markwell M. A., Fox C. F. Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril. Biochemistry. 1978 Oct 31;17(22):4807–4817. doi: 10.1021/bi00615a031. [DOI] [PubMed] [Google Scholar]
  18. McDowell E. M., Becci P. J., Schürch W., Trump B. F. The respiratory epithelium. VII. Epidermoid metaplasia of hamster tracheal epithelium during regeneration following mechanical injury. J Natl Cancer Inst. 1979 Apr;62(4):995–1008. [PubMed] [Google Scholar]
  19. McDowell E. M., Wilson T. S., Trump B. F. Atypical endocrine tumors of the lung. Arch Pathol Lab Med. 1981 Jan;105(1):20–28. [PubMed] [Google Scholar]
  20. Merril C. R., Goldman D., Sedman S. A., Ebert M. H. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 1981 Mar 27;211(4489):1437–1438. doi: 10.1126/science.6162199. [DOI] [PubMed] [Google Scholar]
  21. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  22. Pearse A. G. The cytochemistry and ultrastructure of polypeptide hormone-producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept. J Histochem Cytochem. 1969 May;17(5):303–313. doi: 10.1177/17.5.303. [DOI] [PubMed] [Google Scholar]
  23. Tapia F. J., Polak J. M., Barbosa A. J., Bloom S. R., Marangos P. J., Dermody C., Pearse A. G. Neuron-specific enolase is produced by neuroendocrine tumours. Lancet. 1981 Apr 11;1(8224):808–811. doi: 10.1016/s0140-6736(81)92682-9. [DOI] [PubMed] [Google Scholar]
  24. Yesner R. Spectrum of lung cancer and ectopic hormones. Pathol Annu. 1978;13(Pt 1):207–240. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES