Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1969 Jan;44(1):89–94. doi: 10.1104/pp.44.1.89

Biosynthesis of Fatty Acids by a Soluble Extract From Developing Soybean Cotyledons 1

R W Rinne a
PMCID: PMC396043  PMID: 16657038

Abstract

Fractionation of developing soybean cotyledons into cellular components demonstrates that most of the activity necessary to incorporate acetate-1-14C into lipid remains in the supernatant from a 198,000g spin for 1 hr. The system studied is dependent upon ATP, CoA, and CO2. Concentrations of ATP greater than 4 × 10−3m are inhibitory, while 1 × 10−4m CoA is needed for optimal activity. Avidin inhibition of acetate incorporation into lipid could be reversed by biotin. Studies indicated that NADPH is a better source of reducing power than NADH. The system studied is inhibited by p-chloromercuribenzoic acid and this inhibition can be reversed by an excess of GSH. The system studied shows maximum activity in tris buffer at pH 8.6 or in glycine buffer, pH 9.4.

The distribution of acetate into the various fatty acids is greatly influenced by the temperature of incubation. Cooler incubation temperatures favor the distribution of acetate into the more unsaturated fatty acids.

Full text

PDF
92

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brady R. O. THE ENZYMATIC SYNTHESIS OF FATTY ACIDS BY ALDOL CONDENSATION. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):993–998. doi: 10.1073/pnas.44.10.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Galliard T., Stumpf P. K. Fat metabolism in higher plants. 30. Enzymatic synthesis of ricinoleic acid by a microsomal preparation from developing Ricinus communis seeds. J Biol Chem. 1966 Dec 25;241(24):5806–5812. [PubMed] [Google Scholar]
  3. KATES M., BAXTER R. M. Lipid composition of mesophilic and psychrophilic yeasts (Candida species) as influenced by environmental temperature. Can J Biochem Physiol. 1962 Sep;40:1213–1227. [PubMed] [Google Scholar]
  4. LYNEN F. Biosynthesis of saturated fatty acids. Fed Proc. 1961 Dec;20:941–951. [PubMed] [Google Scholar]
  5. LYNEN F. Participation of acyl--CoA in carbon chain biosynthesis. J Cell Comp Physiol. 1959 Dec;54:33–49. doi: 10.1002/jcp.1030540406. [DOI] [PubMed] [Google Scholar]
  6. MUDD J. B., STUMPF P. K. Fat metabolism in higher plants. XIV. Factors affecting the synthesis of oleic acid by particulate preparations from avocado mesocarp. J Biol Chem. 1961 Oct;236:2602–2609. [PubMed] [Google Scholar]
  7. McMahon V., Stumpf P. K. Fat metabolism in higher plants. XXVI. Biosynthesis of fatty acids in tissues of developing seeds and germinating seedlings of safflower (Carthamus tinctorius L.). Plant Physiol. 1966 Jan;41(1):148–156. doi: 10.1104/pp.41.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. POPJAK G., TIETZ A. Biosynthesis of fatty acids in cell-free preparations. 2. Synthesis of fatty acids from acetate by a soluble enzyme system prepared from rat mammary gland. Biochem J. 1955 May;60(1):147–155. doi: 10.1042/bj0600147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PORTER J. W., WAKIL S. J., TIETZ A., JACOB M. I., GIBSON D. M. Studies on the mechanism of fatty acid synthesis. II. Cofactor requirements of the soluble pigeon liver system. Biochim Biophys Acta. 1957 Jul;25(1):35–41. doi: 10.1016/0006-3002(57)90413-4. [DOI] [PubMed] [Google Scholar]
  10. Squires C. L., Stumpf P. K., Schmid C. Effect of Bicarbonate on the Enzymic Synthesis of Long Chain Fatty Acids. Plant Physiol. 1958 Sep;33(5):365–366. doi: 10.1104/pp.33.5.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. WAKIL S. J., PORTER J. W., GIBSON D. M. Studies on the mechanism of fatty acid synthesis. I. Preparation and purification of an enzymes system for reconstruction of fatty acid synthesis. Biochim Biophys Acta. 1957 Jun;24(3):453–461. doi: 10.1016/0006-3002(57)90233-0. [DOI] [PubMed] [Google Scholar]
  12. WAKIL S. J., TITCHENER E. B., GIBSON D. M. Evidence for the participation of biotin in the enzymic synthesis of fatty acids. Biochim Biophys Acta. 1958 Jul;29(1):225–226. doi: 10.1016/0006-3002(58)90177-x. [DOI] [PubMed] [Google Scholar]
  13. YANG S. F., STUMPF P. K. FAT METABOLISM IN HIGHER PLANTS. XXI. BIOSYNTHESIS OF FATTY ACIDS BY AVOCADO MESOCARP ENZYME SYSTEMS. Biochim Biophys Acta. 1965 Feb 1;98:19–26. [PubMed] [Google Scholar]
  14. Yamada M., Stumpf P. K. Enzymic synthesis of ricinoleic acid by extracts of developing Ricinus communis L. seeds. Biochem Biophys Res Commun. 1964;14:165–171. doi: 10.1016/0006-291x(64)90248-7. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES