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. 1969 Sep;44(9):1291–1294. doi: 10.1104/pp.44.9.1291

Polyribosome Isolation in the Presence of Diethyl Pyrocarbonate 1

Donald P Weeks a, A Marcus a
PMCID: PMC396258  PMID: 5379107

Abstract

Isolation of polyribosomes from wheat embryos and corn root tips in the presence of diethyl pyrocarbonate showed this reagent to have a protective effect on polyribosome structure. In addition, the use of diethyl pyrocarbonate allowed initial homogenization to be performed under less stringent conditions than those normally employed for polyribosome isolation. The use of the reagent is however limited, in that it is deleterious to in vitro ribosomal amino acid incorporation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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