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Plant Physiology logoLink to Plant Physiology
. 1970 Feb;45(2):201–205. doi: 10.1104/pp.45.2.201

Rapid Regeneration of Protochlorophyllide6501

S Granick a, Merrill Gassman b,2
PMCID: PMC396381  PMID: 16657303

Abstract

The rate of regeneration of protochlorophyllide650 was examined spectrophotometrically after a saturating light flash using 8- to 9-day-old dark-grown bean leaves. The regeneration occurred to the extent of 15% with a half rise time of about 20 seconds. Feeding δ-aminolevulinic acid to the excised leaves in the dark increased protochlorophyllides635 but not the absorption at 650 nanometers, suggesting that the holochrome was normally saturated with protochlorophyllide and that the holochrome protein was not controlled by the level of protochlorophyllide. After a light flash, the excess protochlorophyllide, formed from exogenous δ-aminolevulinic acid, readily combined to regenerate the 650 nanometer absorbing species; the regeneration occurred to the extent of 60 to 80% with a half rise time of about 50 seconds. Regeneration was blocked at 0°, suggesting that there was some enzymic process required for regeneration, possibly the formation of a reductant component of the protochlorophyllides650 holochrome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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