Abstract
Soybean plants, Glycine max (L.) Merrill, in standard solution received 2.5 μm ferric ethylenediamine di(o-hydroxyphenylacetate (FeEDDHA) and 0 to 128 μm phosphorus. Their stem exudates contained: 32 to 52 μm Fe, 120 to 5000 μm P, and 120 to 165 μm citrate. Electrophoresis of exudates with high P caused Fe trailing that precluded identification of any major form of Fe. Exudate with low P gave an anodic band of Fe citrate as the major Fe compound. Phosphate added to exudate in vitro depressed the Fe citrate peak and cause Fe trailing. EDDHA added to exudate in vitro pulled Fe from Fe citrate; citrate then migrated as a slower form and Fe migrated as FeEDDHA. A modified preculture system, involving 2-day renewals of 0.2 μm FeEDDHA with 3.2, 9.6, or 16 μm P and low levels of other ions, controlled pH depression and produced considerable change in citrate and P levels. The exudates contained: 45 to 57 μm Fe, 200 to 925 μm P, and 340 to 1025 μm citrate. The high citrate was from plants grown with low P. The major form of Fe in the exudates was Fe citrate. This is probably the form translocated in the plants.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DICKMAN S. R., CLOUTIER A. A. Factors affecting the activity of aconitase. J Biol Chem. 1951 Jan;188(1):379–388. [PubMed] [Google Scholar]
- Rogers C. H., Shive J. W. FACTORS AFFECTING THE DISTRIBUTION OF IRON IN PLANTS. Plant Physiol. 1932 Apr;7(2):227–252. doi: 10.1104/pp.7.2.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tiffin L. O. Iron Translocation II. Citrate/Iron Ratios in Plant Stem Exudates. Plant Physiol. 1966 Mar;41(3):515–518. doi: 10.1104/pp.41.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tiffin L. O. Iron translocation I. Plant culture, exudate sampling, iron-citrate analysis. Plant Physiol. 1966 Mar;41(3):510–514. doi: 10.1104/pp.41.3.510. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tiffin L. O. Translocation of manganese, iron, cobalt, and zinc in tomato. Plant Physiol. 1967 Oct;42(10):1427–1432. doi: 10.1104/pp.42.10.1427. [DOI] [PMC free article] [PubMed] [Google Scholar]