Abstract
Stomata in epidermal strips of Vicia faba opened in light and closed in darkness when floated on dilute K+ solutions. Opening and closing, respectively, paralleled the fluxes of labeled K+ into and out of the strips. The gain and loss of K+ by the strips were shown by colbaltinitrite stain to be centered at guard cells. Intact epidermal cells, however, appeared to take up K+, complicating interpretation of the data.
The specific requirement of K+ for stomatal opening in light appeared to be related to the specific uptake of K+. There was little or no light stimulation of opening in strips on Na+, nor was there stimulation of Na+ uptake. The marked light stimulation of opening on K+ was generally matched by stimulation of K+ uptake.
Anaerobiosis markedly reduced opening in leaf discs but not in strips. Under anaerobic conditions, opening in strips was not appreciably affected by 3(3,4-dichlorophenyl)-1,1-dimethylurea (diuron) but was completely inhibited by carbonyl cyanide m-chlorophenylhydrazone plus diuron. Inhibition of opening was generally correlated with inhibition of K+ uptake by the strips. Also stomata in strips opened well under far red light (>700 nanometers). These data suggest that photosystem I and cyclic electron flow can supply the necessary energy for K+ uptake and stomatal opening.
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