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. 1971 Apr;47(4):581–585. doi: 10.1104/pp.47.4.581

Chromatographic Analysis of a Cytokinin from Tissue Cultures of Crown-Gall 1

John R Tegley a, Francis H Witham a, Michael Krasnuk a
PMCID: PMC396730  PMID: 16657664

Abstract

Extracts from tissue cultures of crown-gall from Parthenocissus tricuspidata (Sieb. and Zucc.) Planch. exhibited cell division activity in the soybean cytokinin assay. The chromatographic migration of one component, responsible for most of the activity, is similar to that of zeatin ribonucleoside. In addition, acid hydrolysis of the active region taken from chromatograms and of an eluate from a cation-exchange resin column resulted in the production of an active free-base-like derivative. Since the derivative and the parent compound lose activity after KMnO4 treatment, they are believed to possess an unsaturated constituent essential for biological activity. The major active factor present in crown-gall from Parthenocissus tricuspidata is therefore distinct from the nicotinamide derivative reported to be present in Vinca rosea L. tumor cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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