Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1971 Jul;48(1):64–68. doi: 10.1104/pp.48.1.64

Plant Nucleases

III. Polyacrylamide Gel Electrophoresis of Corn Ribonuclease Isoenzymes 1

Curtis M Wilson a
PMCID: PMC396802  PMID: 16657737

Abstract

Isoenzymes of RNase were detected in plant extracts after polyacrylamide gel electrophoresis with a new buffer system. The gels were incubated in an RNA solution, then dipped for 30 seconds into 0.2% toluidine blue. The method is rapid and is sensitive to very small amounts of RNase. The effects of buffers and ethylenediaminetetraacetate on the different enzymes are illustrated by photographs and scans of the gels.

RNase I, from endosperms and roots, was the fastest-moving corn RNase. Two isoenzymes of corn RNase II, from microsomes, were detected in the hybrid WF9 × M14, while each parental inbred had one of the isoenzymes. Three isoenzymes of corn Nuclease I, from crude mitochondria, had about the same mobility as the RNase II isoenzymes, but were inhibited by ethylenediaminetetraacetate. RNases were also detected in tobacco and wild carrot tissue cultures.

Full text

PDF
66

Images in this article

65Image
on p.65
65Image
on p.65
65Image
on p.65
66Image
on p.66

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acton G. J., Lewington R. J., Myers A. The effect of light upon ribonuclease activity of etiolated Lupinus albus hypocotyls. Biochim Biophys Acta. 1970 Mar 19;204(1):144–155. doi: 10.1016/0005-2787(70)90497-1. [DOI] [PubMed] [Google Scholar]
  2. Barnard E. A. Ribonucleases. Annu Rev Biochem. 1969;38:677–732. doi: 10.1146/annurev.bi.38.070169.003333. [DOI] [PubMed] [Google Scholar]
  3. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  4. Dalby A., Cagampang G. B. Ribonuclease Activity in Normal, opaque-2, and floury-2 Maize Endosperm during Development. Plant Physiol. 1970 Jul;46(1):142–144. doi: 10.1104/pp.46.1.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dalby A., Davies I. I. Ribonuclease activity in the developing seeds of normal and opaque-2 maize. Science. 1967 Mar 24;155(3769):1573–1575. doi: 10.1126/science.155.3769.1573. [DOI] [PubMed] [Google Scholar]
  6. Ingle J., Beitz D., Hageman R. H. Changes in Composition during Development and Maturation of Maize Seeds. Plant Physiol. 1965 Sep;40(5):835–839. doi: 10.1104/pp.40.5.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lerch B. Phosphodiesterase I. Spezifischer Nachweis nach Disk-Elektrophorese und Vorkommen in Pflanzen. Experientia. 1968 Sep 15;24(9):889–890. doi: 10.1007/BF02138631. [DOI] [PubMed] [Google Scholar]
  8. Randles J. W. Ribonuclease isozymes in Chinese cabbage systemically infected with turnip yellow mosaic virus. Virology. 1968 Dec;36(4):556–563. doi: 10.1016/0042-6822(68)90187-6. [DOI] [PubMed] [Google Scholar]
  9. Reddi K. K. Ribonuclease induction in cells transformed by Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1207–1214. doi: 10.1073/pnas.56.4.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shannon J. C., Hanson J. B., Wilson C. M. Ribonuclease Levels in the Mesocotyl Tissue of Zea mays as a Function of 2,4-Dichlorophenoxyacetic Acid Application. Plant Physiol. 1964 Sep;39(5):804–809. doi: 10.1104/pp.39.5.804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. WILSON C. M. Chromatographic separation of ribonucleases in corn. Biochim Biophys Acta. 1963 Feb 26;68:177–184. doi: 10.1016/0006-3002(63)90133-1. [DOI] [PubMed] [Google Scholar]
  12. Wilson C. M., Alexander D. E. Ribonuclease activity in normal and opaque-2 mutant endosperm of maize. Science. 1967 Mar 24;155(3769):1575–1576. doi: 10.1126/science.155.3769.1575. [DOI] [PubMed] [Google Scholar]
  13. Wilson C. M. Plant nucleases. I. Separation and purification of two ribonucleases and one nuclease from corn. Plant Physiol. 1968 Sep;43(9):1332–1338. doi: 10.1104/pp.43.9.1332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wilson C. M. Purification of a corn ribonuclease. J Biol Chem. 1967 May 10;242(9):2260–2263. [PubMed] [Google Scholar]
  15. Wolf G. Nachweis pflanzlicher Ribonukleasen in Polyacrylamid-Gelen nach Disk-Elektrophorese. Experientia. 1968 Sep 15;24(9):890–891. doi: 10.1007/BF02138632. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES