Abstract
Two forms of protochlorophyllide are found in dark-grown bean (Phaseolus vulgaris, var. Black Velentine) leaves, one (protochlorophyllide650) which is directly photoconvertible to chlorophyllide and another (protochlorophyllide632) which is not. Dark-grown leaves placed in solutions of δ-aminolevulinic acid accumulate protochlorophyllide632. Protochlorophyllide650 and protochlorophyllide632 can be partially separated on sucrose density gradients. A nitrogen atmosphere blocks chlorophyll synthesis in light or the regeneration of protochlorophyllide650 in the dark, even in the presence of excess δ-aminolevulinic acid, except when a stockpile of protochlorophyllide632 is present in the leaf. Under the latter conditions chlorophyll synthesis or protochlorophyllide650 regeneration is accompanied by a decrease in protochlorophyllide632. These experiments suggest that protochlorophyllide632 may be converted to protochlorophyllide650.
Cycloheximide inhibited greening only after an “action-dependent” delay, requiring a predictable minimal period of illumination. This inhibition could be relieved for a time by feeding δ-aminolevulinic acid.
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Selected References
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