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. 1971 Nov;48(5):547–552. doi: 10.1104/pp.48.5.547

Dissociation of N2 Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea 1

C Y Lin a, Joe L Key a
PMCID: PMC396904  PMID: 16657836

Abstract

The dissociation of N2 gas-induced monomeric ribosomes from the pea root was studied by varying the concentration of KCl (or NH4Cl) and MgCl2 in the presence of dithiothreitol. These monoribosomes were shown to dissociate completely into subunits at 0.5m KCl or NH4Cl in the presence of 5 mm MgCl2. The 40S subunits were more susceptible to structural change in KCl than were the 60S subunits. On the other hand, the 60S subunits appeared to be more labile to NH4Cl.

The activity of the subunits relative to aminoacyl-tRNA binding and peptide bond formation was investigated using subunits derived from 0.5 m KCl (or NH4Cl) in the absence and presence of 5 mm MgCl2. The 40S subunits were active in aminoacyl-tRNA binding only when dissociated in the presence of MgCl2. The 40S and 60S subunits combined in the presence of poly U were active in incorporation of 14C-phenylalanine from 14C-phenylalanyl-tRNA only when dissociation was achieved in the presence of 5 mm MgCl2. The KCl-dissociated subunits were much more active in protein synthesis than NH4Cl-dissociated subunits.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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