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. 1971 Dec;48(6):795–800. doi: 10.1104/pp.48.6.795

Membrane Transformations in Aging Potato Tuber Slices 1

Paul A Castelfranco a, Wen-Jing Tang a,2, Marlin L Bolar a,3
PMCID: PMC396951  PMID: 16657883

Abstract

When potato tuber slices (Solanum tuberosum L.) are incubated with radioactive choline, labeled membrane-bound phospholipids are formed. If potato slices are aged for 0 to 24 hours before exposure to radioactive choline, the distribution of the labeled phospholipids undergoes both quantitative and qualitative changes. Quantitatively, there is a marked increase in the total lipoidal radioactivity with aging time. Qualitatively, there is a shift in the kinds of subcellular fractions that are being labeled. Fresh slices incorporate most of the lipoidal radioactivity in the microsomes. Slices aged for 9 hours incorporate most of the label in a fraction consisting of single membrane-bound cisternae, which are presumed to be dictyosomal fragments. Slices aged for 24 hours before incubation with radioactive choline incorporate the greater portion of the label in this same fraction, but a significant portion of the label is found in a heavier, mitochondria-containing fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATTWOOD D., SAUNDEES L. A LIGHT-SCATTERING STUDY OF ULTRASONICALLY IRRADIATED LECITHIN SOLS. Biochim Biophys Acta. 1965 Apr 5;98:344–350. doi: 10.1016/0005-2760(65)90126-8. [DOI] [PubMed] [Google Scholar]
  2. Asahi T., Honda Y., Uritani I. Increase of Mitochondrial Fraction in Sweet Potato Root Tissue after Wounding or Infection with Ceratocystis fimbriata. Plant Physiol. 1966 Sep;41(7):1179–1184. doi: 10.1104/pp.41.7.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLICK R. E., HACKETT D. P. THE ROLE OF PROTEIN AND NUCLEIC ACID SYNTHESIS IN THE DEVELOPMENT OF RESPIRATION IN POTATO TUBER SLICES. Proc Natl Acad Sci U S A. 1963 Aug;50:243–250. doi: 10.1073/pnas.50.2.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dallner G., Siekevitz P., Palade G. E. Biogenesis of endoplasmic reticulum membranes. II. Synthesis of constitutive microsomal enzymes in developing rat hepatocyte. J Cell Biol. 1966 Jul;30(1):97–117. doi: 10.1083/jcb.30.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FLYNN R. M., JONES M. E., LIPMANN F. A colorimetric determination of inorganic pyrophosphate. J Biol Chem. 1954 Dec;211(2):791–796. [PubMed] [Google Scholar]
  6. HERS H. G., BERTHET J., BERTHET L., DE DUVE C. Le système hexose-phosphatasique. III. Localisation intra-cellulaire des ferments par centrifugation fractionnée. Bull Soc Chim Biol (Paris) 1951;33(1-2):21–41. [PubMed] [Google Scholar]
  7. Leaver C. J., Key J. L. Polyribosome formation and RNA synthesis during aging of carrot-root tissue. Proc Natl Acad Sci U S A. 1967 May;57(5):1338–1344. doi: 10.1073/pnas.57.5.1338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MORRE D. J., MOLLENHAUER H. H., CHAMBERS J. E. GLUTARALDEHYDE STABILIZATION AS AN AID TO GOLGI APPARATUS ISOLATION. Exp Cell Res. 1965 Jun;38:672–675. doi: 10.1016/0014-4827(65)90392-7. [DOI] [PubMed] [Google Scholar]
  9. Morré D. J. In vivo incorporation of radioactive metabolites by Golgi apparatus and other cell fractions of onion stem. Plant Physiol. 1970 Jun;45(6):791–799. doi: 10.1104/pp.45.6.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tang W. J., Castelfranco P. A. Phospholipid synthesis in aging potato tuber tissue. Plant Physiol. 1968 Aug;43(8):1232–1238. doi: 10.1104/pp.43.8.1232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Verleur J. D. Studies on the Isolation of Mitochondria from Potato Tuber Tissue. Plant Physiol. 1965 Nov;40(6):1003–1007. doi: 10.1104/pp.40.6.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Verleur J. D., Uritani I. Respiratory Activity of the Mitochondrial Fractions Isolated from Healthy Potato Tubers and from Tuber Tissue Incubated after Cutting or Infection with Ceratocystis fimbriata. Plant Physiol. 1965 Nov;40(6):1008–1012. doi: 10.1104/pp.40.6.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Willemot C., Stumpf P. K. Fat metabolism in higher plants. XXXIV. Development of fatty acid synthetase as a function of protein synthesis in aging potato tuber slices. Plant Physiol. 1967 Mar;42(3):391–397. doi: 10.1104/pp.42.3.391. [DOI] [PMC free article] [PubMed] [Google Scholar]

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