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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Mar;82(6):1852–1854. doi: 10.1073/pnas.82.6.1852

Isolation of morphine from toad skin.

K Oka, J D Kantrowitz, S Spector
PMCID: PMC397372  PMID: 3920656

Abstract

A nonpeptide opioid was found in toad skin and purified to homogeneity by using HPLC with electrochemical detection. A nonpeptide opioid also was detected in bovine brain and adrenals as well as rabbit and rat skin, by reversed-phase HPLC following Sephadex G-15 column chromatography. The material in toad skin was identical to morphine by immunological, pharmacological, and physical chemical criteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blume A. J., Shorr J., Finberg J. P., Spector S. Binding of the endogenous nonpeptide morphine-like compound to opiate receptors. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4927–4931. doi: 10.1073/pnas.74.11.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  3. Gintzler A. R., Levy A., Spector S. Antibodies as a means of isolating and characterizing biologically active substances: presence of a non-peptide, morphine-like compound in the central nervous system. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2132–2136. doi: 10.1073/pnas.73.6.2132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hazum E., Sabatka J. J., Chang K. J., Brent D. A., Findlay J. W., Cuatrecasas P. Morphine in cow and human milk: could dietary morphine constitute a ligand for specific morphine (mu) receptors? Science. 1981 Aug 28;213(4511):1010–1012. doi: 10.1126/science.6267691. [DOI] [PubMed] [Google Scholar]
  5. KUPFERBERG H., BURKHALTER A., WAY E. L. A SENSITIVE FLUOROMETRIC ASSAY FOR MORPHINE IN PLASMA AND BRAIN. J Pharmacol Exp Ther. 1964 Aug;145:247–251. [PubMed] [Google Scholar]
  6. Killian A. K., Schuster C. R., House J. T., Sholl S., Connors M., Wainer B. H. A non-peptide morphine-like compound from brain. Life Sci. 1981 Feb 16;28(7):811–817. doi: 10.1016/0024-3205(81)90165-x. [DOI] [PubMed] [Google Scholar]
  7. Pert C. B., Pasternak G., Snyder S. H. Opiate agonists and antagonists discriminated by receptor binding in brain. Science. 1973 Dec 28;182(4119):1359–1361. doi: 10.1126/science.182.4119.1359. [DOI] [PubMed] [Google Scholar]
  8. Shorr J., Foley K., Spector S. Presence of a non-peptide morphine-like compound in human cerebrospinal fluid. Life Sci. 1978 Nov 13;23(20):2057–2062. doi: 10.1016/0024-3205(78)90239-4. [DOI] [PubMed] [Google Scholar]
  9. Spector S. Quantitative determination of morphine in serum by radioimmunoassay. J Pharmacol Exp Ther. 1971 Aug;178(2):253–258. [PubMed] [Google Scholar]
  10. White M. W. Determination of morphine and its major metabolite, morphine-3-glucuronide, in blood by high-performance liquid chromatography with electrochemical detection. J Chromatogr. 1979 Oct 19;178(1):229–240. doi: 10.1016/s0021-9673(00)89711-3. [DOI] [PubMed] [Google Scholar]

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