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. 1985 Apr;82(7):1954–1958. doi: 10.1073/pnas.82.7.1954

Characterization of a yeast mitochondrial promoter by deletion mutagenesis.

T K Biswas, J C Edwards, M Rabinowitz, G S Getz
PMCID: PMC397457  PMID: 3856873

Abstract

We have generated collections of mutants of the promoter for the small rRNA gene from the mitochondria of yeast deleted from either the 3' or 5' end. Plasmids containing the partially deleted promoter were assayed for their ability to direct correct transcriptional initiation in a homologous in vitro system. We find that the region required for high-efficiency promoter function lies between positions -10 and +2. Our methods detected no effect of flanking sequences on the strength of this promoter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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