Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Apr;82(8):2399–2403. doi: 10.1073/pnas.82.8.2399

Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells.

B M Sutherland, P V Bennett, A G Freeman, S P Moore, P T Strickland
PMCID: PMC397565  PMID: 3857589

Abstract

Neonatal human foreskin fibroblasts can be transformed to anchorage-independent growth by transfection with DNAs inefficient in transforming NIH 3T3 cells. Human cells transfected with DNA from GM 1312, a multiple myeloma cell line, or MOLT-4, a permanent lymphoblast line, grow without anchorage at a much higher frequency than do the parental cells and their DNAs can transform human cell recipients to anchorage-independent growth; they have extended but not indefinite life spans and are nontumorigenic. Human fibroblasts are also transformed by DNAs from two multiple myeloma lines that also transform 3T3 cells; however, restriction analysis suggests that different transforming genes in this DNA are acting in the human and murine systems. These results indicate that the human cell transfection system allows detection of transforming genes not effective in the 3T3 system and points out the possibility of detection of additional transforming sequences even in DNAs that do transform murine cells.

Full text

PDF
2401

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cooper G. M. Cellular transforming genes. Science. 1982 Aug 27;217(4562):801–806. doi: 10.1126/science.6285471. [DOI] [PubMed] [Google Scholar]
  2. Diamond A., Cooper G. M., Ritz J., Lane M. A. Identification and molecular cloning of the human Blym transforming gene activated in Burkitt's lymphomas. Nature. 1983 Sep 8;305(5930):112–116. doi: 10.1038/305112a0. [DOI] [PubMed] [Google Scholar]
  3. Eva A., Tronick S. R., Gol R. A., Pierce J. H., Aaronson S. A. Transforming genes of human hematopoietic tumors: frequent detection of ras-related oncogenes whose activation appears to be independent of tumor phenotype. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4926–4930. doi: 10.1073/pnas.80.16.4926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kakunaga T. Neoplastic transformation of human diploid fibroblast cells by chemical carcinogens. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1334–1338. doi: 10.1073/pnas.75.3.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Land H., Parada L. F., Weinberg R. A. Cellular oncogenes and multistep carcinogenesis. Science. 1983 Nov 18;222(4625):771–778. doi: 10.1126/science.6356358. [DOI] [PubMed] [Google Scholar]
  6. Lane M. A., Sainten A., Cooper G. M. Stage-specific transforming genes of human and mouse B- and T-lymphocyte neoplasms. Cell. 1982 Apr;28(4):873–880. doi: 10.1016/0092-8674(82)90066-6. [DOI] [PubMed] [Google Scholar]
  7. Namba M., Nishitani K., Kimoto T. Carcinogenesis in tissue culture. 29: Neoplastic transformation of a normal human diploid cell strain, WI-38, with Co-60 gamma rays. Jpn J Exp Med. 1978 Aug;48(4):303–311. [PubMed] [Google Scholar]
  8. Peehl D. M., Stanbridge E. J. Anchorage-independent growth of normal human fibroblasts. Proc Natl Acad Sci U S A. 1981 May;78(5):3053–3057. doi: 10.1073/pnas.78.5.3053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pulciani S., Santos E., Lauver A. V., Long L. K., Robbins K. C., Barbacid M. Oncogenes in human tumor cell lines: molecular cloning of a transforming gene from human bladder carcinoma cells. Proc Natl Acad Sci U S A. 1982 May;79(9):2845–2849. doi: 10.1073/pnas.79.9.2845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sager R., Tanaka K., Lau C. C., Ebina Y., Anisowicz A. Resistance of human cells to tumorigenesis induced by cloned transforming genes. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7601–7605. doi: 10.1073/pnas.80.24.7601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Santos E., Tronick S. R., Aaronson S. A., Pulciani S., Barbacid M. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature. 1982 Jul 22;298(5872):343–347. doi: 10.1038/298343a0. [DOI] [PubMed] [Google Scholar]
  12. Shin S. I., Freedman V. H., Risser R., Pollack R. Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4435–4439. doi: 10.1073/pnas.72.11.4435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sutherland B. M., Bennett P. V. Transformation of human cells by DNA transfection. Cancer Res. 1984 Jul;44(7):2769–2772. [PubMed] [Google Scholar]
  14. Sutherland B. M., Oliver R. Culture conditions affect photoreactivating enzyme levels in human fibroblasts. Biochim Biophys Acta. 1976 Sep 6;442(3):358–367. doi: 10.1016/0005-2787(76)90310-5. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES