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. 1985 May;82(9):2574–2578. doi: 10.1073/pnas.82.9.2574

Replication initiator protein of plasmid R6K autoregulates its own synthesis at the transcriptional step.

W Kelley, D Bastia
PMCID: PMC397606  PMID: 3857600

Abstract

The replication initiator protein of plasmid R6K preferentially repressed transcription initiated in vitro from the promoter of the initiator protein cistron. DNase I protection experiments revealed that the sequences in the region of the promoter recognized by the initiator protein partially overlapped the sequences of the same promoter recognized by RNA polymerase of Escherichia coli. Competitive DNase I protection experiments revealed that the initiator not only prevented the RNA polymerase from binding to the promoter sequence but also displaced RNA polymerase from preformed enzyme-promoter binary complexes. Thus, the initiator protein acts as a transcriptional repressor of its own cistron by either preventing RNA polymerase from binding to the promoter or by displacing RNA polymerase from promoter-enzyme complexes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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