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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 May;82(10):3291–3295. doi: 10.1073/pnas.82.10.3291

Interleukin 3 promotes erythroid burst formation in "serum-free" cultures without detectable erythropoietin.

J W Goodman, E A Hall, K L Miller, S G Shinpock
PMCID: PMC397761  PMID: 3923475

Abstract

Erythroid burst-forming units (BFU-E) from mouse bone marrow were grown for 7 days in agar or serum-free methylcellulose cultures in the presence or absence of erythropoietin (Ep) and/or interleukin 3 (IL-3). It was found that IL-3, even in the absence of serum and detectable Ep, was able to stimulate the full development of many erythroid bursts. This IL-3 effect was cell-dose dependent and did not appear to correlate with Ep dose. Spontaneous bursts and those stimulated by Ep only were rare and when seen were very small relative to those produced by IL-3 or IL-3 plus Ep. When addition of IL-3 or Ep to 7-day cultures was delayed, IL-3 but not Ep was shown to maintain BFU-E. No evidence was found by radioimmunoassay that Ep was produced or released in 7-day, "serum-free" cultures of bone marrow nor was Ep activity detected in culture media except those to which it had been added deliberately.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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