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. 1985 May;82(10):3365–3369. doi: 10.1073/pnas.82.10.3365

Generation of a dominant 8-MDa deletion at the left terminus of vaccinia virus DNA.

E Paez, S Dallo, M Esteban
PMCID: PMC397776  PMID: 2987924

Abstract

Vaccinia virus mutants were obtained in high frequency from mouse Friend erythroleukemia (FEL) cells persistently infected with this virus, which contains a large (122-MDa) DNA. During long-term cell passages viral particles with deletions of the DNA are generated in FEL cells. These mutants have a major 8-MDa deletion starting between 2.2 and 3.2 MDa from the left terminus of the viral genome. More than half of the left end terminal repetition is deleted. These mutants have reduced infectivity compared to wild-type virus. The ease with which vaccinia virus mutants are obtained in FEL cells should provide a suitable system for generating mutants with other poxvirus and permit study of the genetic basis of virulence for this group of viruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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