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. 1985 May;82(10):3400–3404. doi: 10.1073/pnas.82.10.3400

Efficient generation of antibodies to oncoproteins by using synthetic peptide antigens.

T Tanaka, D J Slamon, M J Cline
PMCID: PMC397783  PMID: 3858828

Abstract

To examine the efficiency of generating protein-reactive antipeptide antibodies, 35 peptides encoded by retroviral or cellular oncogenes were used to immunize rabbits. Thirty-two peptides elicited antipeptide antibodies, of which 56% reacted with their respective oncoproteins. The length of the immunizing peptide was an important factor in generating antibodies reactive with native protein. Similar peptides differing in a single or a few amino acids could elicite antisera of markedly different reactivities.

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Selected References

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