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. 1985 Jun;82(11):3683–3687. doi: 10.1073/pnas.82.11.3683

Nitrate storage and retrieval in Beta vulgaris: Effects of nitrate and chloride on proton gradients in tonoplast vesicles

Eduardo Blumwald 1, Ronald J Poole 1
PMCID: PMC397851  PMID: 16593570

Abstract

The fluorescent probes acridine orange and oxonol-V were used as indicators of pH gradients (ΔpH) and membrane potential differences (ΔΨ), respectively, in membrane vesicles believed to be derived from the tonoplast of Beta vulgaris L. Low concentrations of nitrate (1-5 mM) caused a partial dissipation of both ΔpH and ΔΨ at vesicle transport sites distinct from the H+-ATPase. In contrast, chloride dissipated only ΔΨ. A model is proposed in which nitrate and chloride enter the plant cell vacuole in response to a potential generated by the tonoplast H+-ATPase. Nitrate but not chloride may then be retrieved for metabolic use by the operation of a nitrate/proton symport at the tonoplast.

Keywords: transport, vacuoles, fluorescence quenching, nitrate, proton symport, chloride

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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