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. 1985 Jun;82(12):3969–3972. doi: 10.1073/pnas.82.12.3969

Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: Evidence for synthesis and processing of a polyprotein in potyvirus genome expression*

Richard F Allison †, John C Sorenson ‡, Mary E Kelly §, Frank B Armstrong §, William G Dougherty †,
PMCID: PMC397915  PMID: 16593574

Abstract

The nucleotide sequence of the 3′-terminal portion of the tobacco etch virus (TEV) genome was determined. The 2324-nucleotide sequence represented approximately one-fourth of the TEV genome and included the capsid protein gene and flanking regions. An open reading frame of 2135 nucleotides and an untranslated region of 189 nucleotides adjacent to a polyadenylate tract were identified. The sequence began within an open reading frame, indicating that the initiation codon was upstream of the available sequence data. The sequence of the 20 NH2-terminal amino acids of the TEV capsid protein was established chemically. An identical amino acid sequence, predicted from the nucleotide sequence, was located, commencing at amino acid - 263. These data indicated that maturation of the capsid protein required a post-translational cleavage of a larger protein precursor, with a probable cleavage site between the amino acids glutamine and glycine.

Keywords: plant virus, RNA, proteolytic cleavage

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahlquist P., Kaesberg P. Determination of the length distribution of poly(A) at the 3' terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA. Nucleic Acids Res. 1979 Nov 10;7(5):1195–1204. doi: 10.1093/nar/7.5.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beachy R. N., Zaitlin M., Bruening G., Israel H. W. A genetic map for the cowpea strain on TMV. Virology. 1976 Sep;73(2):498–507. doi: 10.1016/0042-6822(76)90411-6. [DOI] [PubMed] [Google Scholar]
  3. Ghosh A., Rutgers T., Ke-Qiang M., Kaesberg P. Characterization of the coat protein mRNA of southern bean mosaic virus and its relationship to the genomic RNA. J Virol. 1981 Jul;39(1):87–92. doi: 10.1128/jvi.39.1.87-92.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goelet P., Lomonossoff G. P., Butler P. J., Akam M. E., Gait M. J., Karn J. Nucleotide sequence of tobacco mosaic virus RNA. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5818–5822. doi: 10.1073/pnas.79.19.5818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hari V., Siegel A., Rozek C., Timberlake W. E. The RNA of tobacco etch virus contains poly(A). Virology. 1979 Jan 30;92(2):568–571. doi: 10.1016/0042-6822(79)90159-4. [DOI] [PubMed] [Google Scholar]
  6. Hewick R. M., Hunkapiller M. W., Hood L. E., Dreyer W. J. A gas-liquid solid phase peptide and protein sequenator. J Biol Chem. 1981 Aug 10;256(15):7990–7997. [PubMed] [Google Scholar]
  7. Hiebert E., McDonald J. G. Characterization of some proteins associated with viruses in the potato Y group. Virology. 1973 Nov;56(1):349–361. doi: 10.1016/0042-6822(73)90311-5. [DOI] [PubMed] [Google Scholar]
  8. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., van der Werf S. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature. 1981 Jun 18;291(5816):547–553. doi: 10.1038/291547a0. [DOI] [PubMed] [Google Scholar]
  9. Kurtz D. T., Nicodemus C. F. Cloning of alpha 2u globulin cDNA using a high efficiency technique for the cloning of trace messenger RNAs. Gene. 1981 Mar;13(2):145–152. doi: 10.1016/0378-1119(81)90003-2. [DOI] [PubMed] [Google Scholar]
  10. Lomonossoff G. P., Shanks M. The nucleotide sequence of cowpea mosaic virus B RNA. EMBO J. 1983;2(12):2253–2258. doi: 10.1002/j.1460-2075.1983.tb01731.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Palmenberg A. C., Kirby E. M., Janda M. R., Drake N. L., Duke G. M., Potratz K. F., Collett M. S. The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region. Nucleic Acids Res. 1984 Mar 26;12(6):2969–2985. doi: 10.1093/nar/12.6.2969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rice C. M., Strauss J. H. Synthesis, cleavage and sequence analysis of DNA complementary to the 26 S messenger RNA of Sindbis virus. J Mol Biol. 1981 Aug 15;150(3):315–340. doi: 10.1016/0022-2836(81)90550-7. [DOI] [PubMed] [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Simmons D. T., Strauss J. H. Replication of Sindbis virus. V. Polyribosomes and mRNA in infected cells. J Virol. 1974 Sep;14(3):552–559. doi: 10.1128/jvi.14.3.552-559.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tinoco I., Jr, Borer P. N., Dengler B., Levin M. D., Uhlenbeck O. C., Crothers D. M., Bralla J. Improved estimation of secondary structure in ribonucleic acids. Nat New Biol. 1973 Nov 14;246(150):40–41. doi: 10.1038/newbio246040a0. [DOI] [PubMed] [Google Scholar]
  16. Zimmern D., Kaesberg P. 3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4257–4261. doi: 10.1073/pnas.75.9.4257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. van Wezenbeek P., Verver J., Harmsen J., Vos P., van Kammen A. Primary structure and gene organization of the middle-component RNA of cowpea mosaic virus. EMBO J. 1983;2(6):941–946. doi: 10.1002/j.1460-2075.1983.tb01525.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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