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. 1985 Jun;82(12):4268–4269. doi: 10.1073/pnas.82.12.4268

Failure to demonstrate pluripotential hemopoietic stem cells in mouse brains.

P M Hoogerbrugge, G Wagemaker, D W van Bekkum
PMCID: PMC397978  PMID: 3858879

Abstract

Hemopoietic stem cells as defined by the capacity to produce spleen colonies in lethally irradiated recipients were reported by P. F. Bartlett [(1982) Proc. Natl. Acad. Sci. USA 79, 2722-2725] to be present in high frequencies in mouse brain. He also reported similar numbers of colony-forming units, spleen (CFU-s), in the brains of Wf/Wf mice, the bone marrow of which lacks detectable spleen colony-forming cells. To verify these observations, single cell suspensions were produced from murine brains by incubation with trypsin and DNase, followed by removal of myelin by Percoll gradient centrifugation. Two to 13 CFU-s were detected per brain. This low number suggested contamination of the brains by either blood or bone marrow leaking from the skull bones during dissection. When the isolated, intact brains were washed carefully in balanced salt solution, the recovered number of CFU-s decreased to 0.1-0.4 per brain. No CFU-s could be detected in the brains of W/Wv mice. It is concluded that the CFU-s observed by Bartlett in preparations of mouse brain did not originate from the brain tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartlett P. F. Pluripotential hemopoietic stem cells in adult mouse brain. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2722–2725. doi: 10.1073/pnas.79.8.2722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ferrer I., Sarmiento J. Reactive microglia in the developing brain. Acta Neuropathol. 1980;50(1):69–76. doi: 10.1007/BF00688538. [DOI] [PubMed] [Google Scholar]
  3. Ganick D. J., Sondel P. M., Gilbert E. F., Borcherding W. Leukemia presenting as central nervous disease without bone marrow involvement. Med Pediatr Oncol. 1983;11(4):229–232. doi: 10.1002/mpo.2950110404. [DOI] [PubMed] [Google Scholar]
  4. Guenet J. L., Marchal G., Milon G., Tambourin P., Wendling F. Fertile dominant spotting in the house mouse. J Hered. 1979 Jan-Feb;70(1):9–12. doi: 10.1093/oxfordjournals.jhered.a109202. [DOI] [PubMed] [Google Scholar]
  5. Kerns J. M., Hinsman E. J. Neuroglial response to sciatic neurectomy. I. Light microscopy and autoradiography. J Comp Neurol. 1973 Oct 1;151(3):237–254. doi: 10.1002/cne.901510303. [DOI] [PubMed] [Google Scholar]
  6. Letendre L., Banks P. M., Reese D. F., Miller R. H., Scanlon P. W., Kiely J. M. Primary lymphoma of the central nervous system. Cancer. 1982 Mar 1;49(5):939–943. doi: 10.1002/1097-0142(19820301)49:5<939::aid-cncr2820490518>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  7. MCCULLOCH E. A., SIMINOVITCH L., TILL J. E. SPLEEN-COLONY FORMATION IN ANEMIC MICE OF GENOTYPE WW. Science. 1964 May 15;144(3620):844–846. doi: 10.1126/science.144.3620.844. [DOI] [PubMed] [Google Scholar]
  8. Marsh W. L., Jr, Stevenson D. R., Long H. J., 3rd Primary leptomeningeal presentation of T-cell lymphoma. Report of a patient and review of the literature. Cancer. 1983 Mar 15;51(6):1125–1131. doi: 10.1002/1097-0142(19830315)51:6<1125::aid-cncr2820510625>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  9. Mendenhall N. P., Thar T. L., Agee O. F., Harty-Golder B., Ballinger W. E., Jr, Million R. R. Primary lymphoma of the central nervous system. Computerized tomography scan characteristics and treatment results for 12 cases. Cancer. 1983 Dec 1;52(11):1993–2000. doi: 10.1002/1097-0142(19831201)52:11<1993::aid-cncr2820521104>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  10. Rappeport J. M., Ginns E. I. Bone-marrow transplantation in severe Gaucher's disease. N Engl J Med. 1984 Jul 12;311(2):84–88. doi: 10.1056/NEJM198407123110203. [DOI] [PubMed] [Google Scholar]
  11. Skoff R. P. The fine structure of pulse labeled (3-H-thymidine cells) in degenerating rat optic nerve. J Comp Neurol. 1975 Jun 15;161(4):595–611. doi: 10.1002/cne.901610408. [DOI] [PubMed] [Google Scholar]
  12. TILL J. E., McCULLOCH E. A. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res. 1961 Feb;14:213–222. [PubMed] [Google Scholar]

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