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. 1995 Dec 19;92(26):12151–12155. doi: 10.1073/pnas.92.26.12151

Phospholipase D signaling is essential for meiosis.

K Rose 1, S A Rudge 1, M A Frohman 1, A J Morris 1, J Engebrecht 1
PMCID: PMC40314  PMID: 8618862

Abstract

Phospholipid metabolism plays an important role in cellular regulation by generating second messengers for signal transduction. Many stimuli activate a phospholipase D, which catalyzes the hydrolysis of phosphatidylcholine, producing phosphatidic acid and choline. Here we report that the yeast SP014 gene, which is essential for meiosis [Honigberg, S. M., Conicella, C. & Esposito, R. E. (1992) Genetics 130, 703-716], encodes a phospholipase D. SP014 RNA and protein activity are induced during late meiotic prophase, and the enzyme has properties similar to mammalian phosphatidylinositol 4,5-bisphosphate-regulated phospholipase D. Characterization of an unusual allele of SP014 defines regions of the protein important for enzyme catalysis and regulation. These results implicate phospholipase D signaling in regulating cellular differentiation.

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Selected References

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