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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1995 Nov 21;92(24):11264–11268. doi: 10.1073/pnas.92.24.11264

Cell cycle regulation of the cyclin A gene promoter is mediated by a variant E2F site.

A Schulze 1, K Zerfass 1, D Spitkovsky 1, S Middendorp 1, J Bergès 1, K Helin 1, P Jansen-Dürr 1, B Henglein 1
PMCID: PMC40612  PMID: 7479977

Abstract

Cyclin A is involved in the control of S phase and mitosis in mammalian cells. Expression of the cyclin A gene in nontransformed cells is characterized by repression of its promoter during the G1 phase of the cell cycle and its induction at S-phase entry. We show that this mode of regulation is mediated by the transcription factor E2F, which binds to a specific site in the cyclin A promoter. It differs from the prototype E2F site in nucleotide sequence and protein binding; it is bound by E2F complexes containing cyclin E and p107 but not pRB. Ectopic expression of cyclin D1 triggers premature activation of the cyclin A promoter by E2F, and this effect is blocked by the tumor suppressor protein p16INK4.

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Selected References

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