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. 1995 Nov 7;92(23):10477–10481. doi: 10.1073/pnas.92.23.10477

Identification and characterization of a Drosophila nuclear receptor with the ability to inhibit the ecdysone response.

A C Zelhof 1, T P Yao 1, R M Evans 1, M McKeown 1
PMCID: PMC40634  PMID: 7479823

Abstract

In a search for retinoid X receptor-like molecules in Drosophila, we have identified an additional member of the nuclear receptor superfamily, XR78E/F. In the DNA-binding domain, XR78E/F is closely related to the mammalian receptor TR2, as well as to the nuclear receptors Coup-TF and Seven-up. We demonstrate that XR78E/F binds as a homodimer to direct repeats of the sequence AGGTCA. In transient transfection assays, XR78E/F represses ecdysone signaling in a DNA-binding-dependent fashion. XR78E/F has its highest expression in third-instar larvae and prepupae. These experiments suggest that XR78E/F may play a regulatory role in the transcriptional cascade triggered by the hormone ecdysone in Drosophila.

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