Abstract
Lower genital tract infection with Chlamydia trachomatis and C. muridarum can induce long-lasting hydrosalpinx in the upper genital tract of women and female mice, respectively. However, A/J mice were highly resistant to induction of long-lasting hydrosalpinx by C. muridarum. We further compared host inflammatory responses and chlamydial infection courses between the hydrosalpinx-resistant A/J mice and CBA/J mice known to be susceptible to hydrosalpinx induction. Both mouse strains developed robust pyosalpinx during the acute phase followed by hydrosalpinx during the chronic phase. However, the hydrosalpinges disappeared in A/J mice by day 60 after infection, suggesting that some early hydrosalpinges are reversible. Although the overall inflammatory responses were indistinguishable between CBA/J and A/J mice, we found significantly more neutrophils in oviduct lumen of A/J mice on days 7 and 10, which correlated with a rapid but transient oviduct invasion by C. muridarum with a peak infection on day 7. In contrast, CBA/J mice developed a delayed and extensive oviduct infection. These comparisons have revealed an important role of the interactions of oviduct infection with inflammatory responses in chlamydial induction of long-lasting hydrosalpinx, suggesting that a rapid but transient invasion of oviduct by chlamydial organisms can prevent the development of the long-lasting hydrosalpinges.
INTRODUCTION
Long-lasting hydrosalpinx is a pathological hallmark of tubal factor infertility associated with Chlamydia trachomatis infection in women (1) and C. muridarum infection in female mice (2–4). The precise mechanism of Chlamydia-induced long-lasting hydrosalpinx remains unknown, although Chlamydia-triggered inflammatory responses have been hypothesized to contribute significantly to chlamydial pathogenesis (5, 6). For example, it is not clear whether live organism infection in the fallopian tube is necessary for induction of hydrosalpinx, how chlamydial organisms trigger hydrosalpinx-causing inflammation, and when hydrosalpinx becomes irreversible. Since it is difficult to directly address these questions during C. trachomatis infection in women, a murine model of C. muridarum infection has been used extensively to study pathogenic mechanisms and immune responses of C. trachomatis (7, 8). This is because genital tract infection of mice with C. muridarum can lead to long-lasting hydrosalpinx that closely mimics hydrosalpinx found in C. trachomatis-infected women under laparoscopy (9–11).
To delineate the host inflammatory mechanism of chlamydial induction of long-lasting hydrosalpinx, Shah et al. carefully compared the genital tract inflammatory pathological responses between the chlamydial infection-resistant C57BL/6J and the susceptible C3H/HeN mice, which led the authors to correlate a robust acute inflammatory response with the development of long-lasting hydrosalpinx observed on day 56 after infection (3). However, it is not known whether the intensity of the acute inflammatory response can always contribute to chlamydial induction of long-lasting hydrosalpinx, since both C57BL/6L and C3H/HeN can develop significant long-lasting hydrosalpinx, although with different incidence rates (3, 12). It is also unclear whether live organism infection in the oviduct is necessary for sustaining hydrosalpinx-causing inflammation, since live organism recovery was monitored in the lower but not upper genital tract (3). Darville et al. further reported that mice deficient in TLR2 failed to develop a robust acute inflammatory response with decreased oviduct dilation (13). However, these observations were made under a microscope on day 35 after infection (13). It is not clear whether the proposed TLR2-mediated signaling pathway is sufficient for C. muridarum induction of long-lasting hydrosalpinx. More importantly, the TLR2 knockout mice developed an equivalent level of chronic inflammation in the oviduct on day 35 after infection (13), and mice deficient in MyD88, a critical adaptor molecule of the TLR2-mediated signaling pathway, developed more severe long-lasting hydrosalpinx (14). These findings question the role of TLR2 signaling pathways in chlamydial induction of long-lasting hydrosalpinx. The role of neutrophils in chlamydial infection and pathogenesis has been extensively studied. While some found a protective role of early neutrophil infiltration in protection against chlamydial pathogenicity (15, 16), others correlated an increased infiltration of neutrophils with chlamydial pathogenicity (17). Many other host molecules have been shown to contribute to chlamydial pathogenicity in the upper genital tract, including matrix metalloproteinases (18), inducible nitric oxide synthase (19), interleukin-1 (IL-1) receptor (20), caspase-1 (6, 21), IL-17 (16), CD28 (22), and tumor necrosis factor alpha (TNF-α) (23). However, none of these previous studies have sufficiently addressed the questions on the mechanism, location, duration, and extent of inflammatory signaling pathways activated during chlamydial infection as they influence chlamydial induction of long-lasting hydrosalpinx. Recently, we correlated the live organism infection in the oviduct with chlamydial induction of long-lasting hydrosalpinx (12), which is consistent with the observation that epithelial cells actively infected with chlamydial organisms are more inflammatory than cells stimulated with noninfectious chlamydial antigens (5, 24, 25). The current study extends our previous observations by further defining the relationship between oviduct infection and oviduct inflammatory responses in chlamydial pathogenesis.
In the current study, we unexpectedly found that A/J mice were highly resistant to induction of long-lasting hydrosalpinx by C. muridarum. We took advantage of this mouse property and carefully compared its host inflammatory responses and chlamydial infection courses with that of CBA/J mice that are known to be susceptible to hydrosalpinx induction (12). We found that both mouse strains developed robust pyosalpinx during the acute phase followed by hydrosalpinx during the chronic phase. However, in A/J mice, the hydrosalpinges disappeared by day 60 after infection, demonstrating that some early hydrosalpinges are reversible. Although the overall inflammatory responses were indistinguishable between CBA and A/J mice, an accelerated exudation of neutrophils into the oviduct lumen coupled with a rapid but transient oviduct infection within the first week after intravaginal inoculation was identified in the hydrosalpinx-resistant A/J mice. In contrast, a more extensive oviduct infection beyond 2 weeks after intravaginal inoculation was found in the hydrosalpinx-susceptible CBA/J mice. These observations have revealed an important relationship between oviduct infection and inflammatory responses in chlamydial induction of long-lasting hydrosalpinx, correlating a rapid but transient invasion of oviduct by chlamydial organisms followed by an enhanced neutrophil exudation with the prevention of the irreversible hydrosalpinges.
MATERIALS AND METHODS
Chlamydial organisms and infection.
Chlamydia muridarum (Nigg strain) organisms used in the current study were propagated in HeLa cells (human cervical carcinoma epithelial cells; ATCC catalog no. CCL2.1), purified, aliquoted, and stored as described previously (26). Female CBA/J (stock number 000656) and A/J mice (000646) were purchased at the age of 5 to 6 weeks old from Jackson Laboratories (Bar Harbor, ME). Each mouse was inoculated intravaginally with 2 × 105 inclusion-forming units (IFUs) of C. muridarum organisms in 20 μl of SPG (sucrose-phosphate-glutamate buffer). Five days prior to infection, each mouse was injected subcutaneously with 2.5 mg depot medroxyprogesterone acetate (Depo-Provera; Pharmacia Upjohn, Kalamazoo, MI) to synchronize the estrus cycle and increase mouse susceptibility to chlamydial infection. For in vitro infection of HeLa cells, HeLa cells grown on coverslips in 24-well plates containing Dulbecco's modified Eagle medium (DMEM) (GIBCO BRL, Rockville, MD) with 10% fetal calf serum (FCS; GIBCO BRL) at 37°C in an incubator supplied with 5% CO2 were inoculated with C. muridarum organisms as described previously (6). The infected cultures were examined by immunofluorescence as described below.
Monitoring live C. muridarum organism recovery from swabs and genital tract tissues.
To monitor live organism shedding, vaginal swabs were taken on different days after intravaginal infection. Each swab was suspended in 500 μl of ice-cold SPG followed by vortexing with glass beads, and the released organisms were titrated on HeLa cell monolayers in duplicates as described previously (14). To monitor upper genital tract infection, the genital tract tissue was harvested sterilely from each mouse on different days after infection as indicated in individual experiments. Each tissue was cut into 3 segments, including vagina/cervix, uterus/uterine horn (both sides from the same mouse were combined as a single segment), and oviducts/ovaries (both sides from the same mouse were pooled as a single tissue sample). Each segment sample was homogenized in 300 μl of SPG using a 2-ml mini tissue grinder (Fisher Scientific, Pittsburgh, PA). After brief sonication, the released live organisms were titrated as described above. The total number of IFUs per swab/tissue was calculated based on the number of IFUs per field, number of fields per coverslip, dilution factors, and inoculation and total sample volumes. An average was taken from the serially diluted and duplicate samples for any given swab/tissue. The calculated total number of IFUs/swab or tissue was converted into log10 values, and the log10 IFUs were used to calculate means and standard deviations for each group at each time point.
Evaluating mouse genital tract tissue pathologies and histological scoring.
Mice were sacrificed on different days postinfection as indicated in individual experiments, and urogenital tract tissues were isolated. Before the tissues were removed, in situ gross examination was performed for evidence of oviduct pathologies, including pyosalpinx and hydrosalpinx. Mice with pyosalpinx or hydrosalpinx on either side of oviducts were determined to be positive when calculating the percentage of mice with positive oviduct pathology. The severity of hydrosalpinx was scored based on the following criteria: 0, no pyosalpinx or hydrosalpinx; 1, pyosalpinx or hydrosalpinx barely visible, requiring confirmation under stereoscope or microscope examination; 2, pyosalpinx or hydrosalpinx clearly visible by the naked eye but smaller in size than the ovary on the same side; 3, pyosalpinx or hydrosalpinx size equal to that of the ovary on the same side; and 4, pyosalpinx or hydrosalpinx size larger than that of the ovary on the same side. Scores from both sides of oviducts from the same mouse were combined as the score for that mouse. Obviously, both hydrosalpinx incidence rates and hydrosalpinx severity scores were determined by the naked eye by observing the isolated genital tract organs. Thus, they represent gross pathology.
For histological scoring and inflammatory cell counting, the excised mouse genital tract tissues, after photographing, were fixed in 10% neutral formalin, embedded in paraffin, and serially sectioned longitudinally (with 5 μm/each section). Efforts were made to include cervix, both uterine horns, and oviducts, as well as lumenal structures of each tissue in each section. The sections were stained with hematoxylin and eosin (H&E) as described elsewhere (3). The H&E-stained sections were scored for severity of inflammation and pathologies based on the modified schemes established previously (3, 14) by board-certified pathologists who were blinded as to mouse group designation. The following values were used for scoring the dilatation of the oviduct: 0, no significant dilatation; 1, mild dilatation of a single cross-section; 2, one to three dilated cross-sections; 3, more than three dilated cross-sections; and 4, confluent pronounced dilation. Inflammatory cell infiltrates were scored for oviduct lumen and interstitial tissue (oviduct wall tissue) separately: 0, no significant infiltration; 1, infiltration at a single focus; 2, infiltration at two to four foci; 3, infiltration at more than four foci; and 4, confluent infiltration. Scores from both sides of the oviducts were added to represent the oviduct pathology for a given mouse, and the individual mouse scores were calculated as medians for each group. Thus, oviduct lumenal dilation scores and oviduct lumen or wall tissue inflammation scores represent microscopic observations. Together with the gross pathology parameters hydrosalpinx incidence and severity, the combination of the 4 parameters allow us to more accurately describe the oviduct pathology.
In some experiments, the individual inflammatory cells were identified and counted from oviduct lumenal and wall tissue areas. For example, both total inflammatory cells and neutrophils and mononuclear cells were counted in individual 100× objective lens views. Inflammatory cells from 5 to 10 random 100× objective lens views were counted from each oviduct cross-section from both sides. An average number of total and individual inflammatory cells per view from each mouse was used to calculate mouse group means and standard deviations.
Immunofluorescence assay.
HeLa cells grown on coverslips with chlamydial infection were fixed and permeabilized for immunostaining as described previously (27, 28). Hoechst dye (blue; Sigma) was used to visualize nuclear DNA. For titrating IFUs from swab and tissue homogenate samples, a mouse antichlamydial lipopolysaccharide (LPS) antibody (clone MB5H9; unpublished observations) plus a goat anti-mouse IgG conjugated with Cy3 (red; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial inclusions. Hoechst dye (Sigma-Aldrich, St. Louis, MO) was used to label DNA (blue). For detecting C. muridarum inclusions in mouse genital tract tissue sections, a rabbit anti-C. muridarum antibody (raised with purified C. muridarum elementary bodies; unpublished data) was used to label chlamydial inclusions followed by a goat anti-rabbit IgG conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc.). All immunofluorescence-labeled samples were observed under an Olympus AX-70 fluorescence microscope (Olympus, Melville, NY). The images were taken using a Simple PCI and processed using Adobe Photoshop as described previously (29, 30).
Statistical analyses.
The differences in IFUs recovered from mouse swabs and tissues and differences in inclusion and inflammatory cell counting were analyzed using Kruskal-Wallis or analysis of variance (ANOVA) followed by Student t test. The pathology scores (both gross and microscopic) were analyzed with a Wilcoxon rank-sum test. Fisher's exact test was used to analyze category data, including incidence rates.
RESULTS
Failure of A/J mice to develop long-lasting hydrosalpinx following C. muridarum intravaginal infection.
We previously reported that five strains of mice, including CBA/J, were induced to develop long-lasting hydrosalpinx upon intravaginal infection with C. muridarum (12). When we tested A/J mice, the result was unexpected. The A/J mice were highly resistant to hydrosalpinx induction (Fig. 1). With CBA/J mice as a control, both strains (n = 10 for CBA/J and n = 10 for A/J) were sacrificed for observing hydrosalpinx on day 60 after intravaginal infection with C. muridarum. A/J mice failed to develop any significant long-lasting hydrosalpinx. Both hydrosalpinx incidence rates (Fisher's exact test) and severity scores (Wilcoxon rank-sum test) were significantly lower in A/J than CBA/J mice (P < 0.01 for both).
Early pyohydrosalpinges in both CBA/J and A/J but long-lasting hydrosalpinx only in CBA/J mice.
To investigate the mechanism of Chlamydia-induced hydrosalpinx, we compared oviduct gross pathology following C. muridarum infection in both CBA/J and A/J mice (Fig. 2). Mice infected with C. muridarum were sacrificed for visual observation of pyosalpinx and hydrosalpinx on days 3, 7, 10, 14, 21, 28, 35, and 60 after infection. Pyosalpinx and hydrosalpinx were visually identified in both CBA/J and A/J mice. These gross pathologies were validated under a microscope. Oviducts with pyosalpinx were filled with inflammatory infiltrates, while those with hydrosalpinx showed empty lumenal space suggestive of clear solution accumulation. Note that inflammation infiltration was also observed under microscopy in some oviducts without visually detectable pathology. Obviously, microscopic observation is more sensitive than visual observation. No oviduct gross pathology was detected in mice sacrificed on days 3 and 7. Thus, images from these 2 time points were not included. Both the gross pathology incidence rates and severity scores were determined for each mouse and calculated from each group at each time point (Fig. 3). Extensive pyosalpinx was observed in both strains of mice during the first 4 weeks after infection, followed by or overlapping hydrosalpinx in both strains of mice. However, by day 60, no significant hydrosalpinx was detected in A/J mice.
Rapid neutrophil recruitment into oviduct lumen of A/J during acute phase.
We first compared the overall inflammatory infiltration in oviduct lumen and tissues separately between CBA/J and A/J mice (Fig. 4). The inflammatory scores were largely similar between these two strains of mice along the entire infection course. The oviduct lumenal inflammatory scores peaked on day 14, while the tissue scores peaked on days 10 and 14 after infection. The only significant differences between CBA/J and A/J mice were the tissue inflammatory scores on day 60. There was no overall significant difference. These observations suggest that the microscopic assessment of overall inflammation infiltration was not sensitive enough for identifying correlates associated with either hydrosalpinx resistance or susceptibility.
We then counted individual types of inflammatory cells, including neutrophils and mononuclear cells (mainly consisting of macrophages, lymphocytes, and plasma cells), in both oviduct lumen and tissue sections (Fig. 5). Under a 100× objective lens, neutrophils and mononuclear cells can be distinguished. We found that during the first 10 days of infection, most inflammatory cells from either oviduct lumen or tissue sections were neutrophils for both CBA/J and A/J mice. Interestingly, the level of neutrophil exudation in the oviduct lumen was significantly higher in the hydrosalpinx-resistant A/J than the susceptible CBA/J mice on both days 7 and 10. The tissue neutrophil infiltration peaked on day 7, while the lumenal neutrophils peaked on day 10. By day 14, the neutrophils started to drop and continued to decline thereafter, which marked the onset of chronic infection. Consistent with the chronic infection, significant numbers of inflammatory cells became mononuclear cells on day 14. The mononuclear cells continued to increase and peaked on day 28. By day 60, only a minimal number of inflammatory cells was detected in A/J mice, while a significant number of inflammatory cells was still maintained in CBA/J mice, which is consistent with the overall inflammation assessment described above for Fig. 4. Together, the above-described observations demonstrated that the hydrosalpinx-resistant A/J mice responded to chlamydial infection with a more rapid recruitment of neutrophils into the oviduct lumen during the acute phase with significantly more neutrophil infiltration on days 7 and 10 after infection compared to those of the hydrosalpinx-susceptible CBA/J mice. These results suggested a correlation between rapid neutrophil recruitment during the acute phase and lack of long-lasting hydrosalpinx.
Oviduct infection peaks earlier but resolves faster in A/J than CBA/J mice.
Since both infection courses and host responses can contribute to chlamydial induction of hydrosalpinx, we further compared chlamydial infection courses in CBA/J and A/J mice by monitoring the live organism recovery from vaginal swabs following intravaginal inoculation with C. muridarum (Fig. 6). We found that A/J mice displayed a significantly reduced infection time course. The level of live organisms recovered from A/J mouse vaginal swabs dropped significantly on day 14 compared to those of CBA/J mice. All 10 A/J mice cleared infection by day 35, while CBA mice continued to shed live organisms until day 56 after infection. Obviously, the reduced recovery of live organisms from vaginal swabs correlated well with the lack of hydrosalpinx in A/J mice.
However, the vaginal swab infectious organism recovery only measured infection of the lower genital tract, which might not reflect the extent of infection in the upper genital tract where long-lasting hydrosalpinx occurs. We then directly detected chlamydial infection in the upper genital tract (Fig. 7). Using an immunofluorescence assay, we detected significantly more inclusions in the oviduct sections of A/J than CBA/J mice on day 7 after infection. However, the number of inclusions rapidly increased so that those of A/J mice surpassed those of CBA/J mice on day 10 after infection and thereafter. By day 21, chlamydial inclusions were still detectable in the oviduct of CBA/J but not A/J mice. To validate this observation, we quantitated the infectious organisms recovered from oviduct/ovary tissue homogenates in independent experiments. We found that the level of infectious organisms was higher in A/J mice on day 7 but significantly lower on days 14 and 21 compared to levels for CBA/J mice. Infectious particles were still recovered from the oviduct/ovary tissue of CBA/J but not A/J mice by day 28 after infection when early hydrosalpinges became obvious (Fig. 2). These observations together have confirmed that the hydrosalpinx-resistant A/J mice experienced a rapid but transient oviduct infection while the hydrosalpinx-susceptible CBA/J mice developed a slower but more extended oviduct infection.
DISCUSSION
Our unexpected discovery of A/J mice as being highly resistant to induction of long-lasting hydrosalpinx by C. muridarum has provided us a unique tool for further investigating the mechanisms of chlamydial pathogenic mechanisms in the murine model. By simultaneously comparing host inflammatory responses and chlamydial infection courses between the hydrosalpinx-resistant A/J and CBA/J mice known to be susceptible to hydrosalpinx induction, we have further defined the relationship between oviduct infection and inflammatory responses in chlamydial pathogenesis. Although both mouse strains developed robust pyosalpinx during the acute phase followed by hydrosalpinx during the chronic phase, the hydrosalpinges disappeared in A/J mice by day 60 after infection. The lack of long-lasting hydrosalpinx in A/J mice correlated with an accelerated exudation of neutrophils into the oviduct lumen during early stages of infection. As a result, the A/J mice only experienced a transient oviduct infection. In contrast, the hydrosalpinx-susceptible CBA/J mice developed more extended mononuclear cell infiltration with a more extensive oviduct infection, which may significantly contribute to the development of long-lasting hydrosalpinx.
A second surprise finding in the current study was that the hydrosalpinx-resistant A/J mice also developed robust pyosalpinx and maintained hydrosalpinx during the first 5 weeks. More than 50% of A/J mice developed visible hydrosalpinx by days 28 or 35 after infection. This is certainly an underestimate, since visual observation is not nearly as sensitive as microscope examination for identifying hydrosalpinx. Microscopic examination of the same A/J mouse oviducts validated the hydrosalpinges visually identified. The hydrosalpinges revealed under microscopy were as severe as those previously identified microscopically on day 35 (13) or 42 (31) after infection. However, by day 60 after infection, most of these early hydrosalpinges disappeared in A/J mice. These observations have demonstrated that the hydrosalpinges detected using microscopy during the first 5 or 6 weeks of infection are reversible and do not always develop into long-lasting hydrosalpinx. Indeed, fibrosis is known to be reversible (32). Tubal fibrosis responsible for causing these early hydrosalpinges may be reversed in the late stages of infection. In addition, it has been reported that not all mouse hydrosalpinges represent complete fibrotic blockage of the oviducts (3). However, it is the long-lasting hydrosalpinx that is most medically relevant, since fallopian tubal occlusion is detected in most women with tubal-factor infertility by hysterosalpingography (33). Thus, it is necessary to ensure that the observed hydrosalpinx is long-lasting and represents irreversible tubal blockage when investigating chlamydial pathogenic mechanisms using the C. muridarum murine infection model, which can be achieved by either using a dye solution to directly measure the oviduct patency (3) or visually observing hydrosalpinx 60 days or longer after infection (12, 14, 22, 27, 34, 35).
A third surprise finding in the current manuscript was our observation that significantly more chlamydial inclusions in the oviduct sections and higher numbers of infectious organisms in the homogenate of oviduct/ovary were detected in the hydrosalpinx-resistant A/J mice on day 7 after infection compared to CBA/J mice. This rapid ascension should have made the A/J mice more susceptible to the development of more severe upper genital pathology. Although the A/J mice developed pyosalpinx and maintained hydrosalpinx during the first 5 weeks, by day 60, these early hydrosalpinges disappeared. The question is why? One hypothesis is that the rapid ascension triggered an accelerated acute inflammation, as evidenced by the significantly enhanced neutrophil exudation into oviduct lumen of the A/J mice on days 7 and 10 after infection. The enhanced acute inflammatory neutrophils may be effective in limiting chlamydial infection in the oviduct. This hypothesis is supported by the finding that the rapid oviduct infection peaked on day 7, followed by a significantly suppressed chlamydial organism count on days 10 and 14 and no infectious organisms detectable by day 28. The roles of early neutrophil infiltration in protection against chlamydial infection and pathogenicity have been demonstrated previously (15, 16). Although the significantly reduced oviduct infection may have induced the acute pyosalpinx and early hydrosalpinx, it may not be able to maintain inflammation that drives the development of long-lasting hydrosalpinx. This hypothesis is also supported by our recent findings that long-lasting hydrosalpinx require significant and extended oviduct infection (12). Alternatively, a second hypothesis is that A/J mice are genetically defective in responding to chlamydial infection with a hydrosalpinx-causing prolonged inflammatory response, which is inconsistent with the equally robust pyosalpinx we detected in both A/J and CBA/J mice. Interestingly, A/J mice are known to be deficient in complement component 5 (C5) (36, 37). Since C5 has been found to play significant roles in various inflammatory pathologies and anti-C5 therapy has been shown to reduce the severity of autoimmune pathologies (38, 39), it is possible that A/J mice are not able to develop long-lasting hydrosalpinx if C5 is required for the conversion of early hydrosalpinx into long-lasting hydrosalpinx. Although complement component 3 (C3) has been shown to be protective in controlling C. muridarum lung infection (40), the role of C5 in either chlamydial infection or pathogenesis has not been evaluated. Since C5 can be activated independently of C3 (41), we are currently examining the role of C5 in chlamydial induction of hydrosalpinx, which may help explain the pronounced pathological differences observed between A/J and CBA/J mice and provide insights in devising effective therapeutic interventions.
Although we have revealed a distinct difference in long-lasting hydrosalpinx development between CBA/J and A/J mice, it is not clear whether these two strains also display such a distinct difference in infertility during mating experiments. Thus, besides using the CBA/J and A/J mouse platform to further understand the cellular and molecular mechanisms involved in hydrosalpinx development, it will be worth testing whether A/J mice can also maintain largely normal fertility 60 days after C. muridarum infection when CBA/J mice are induced to develop significant infertility.
Footnotes
Published ahead of print 7 April 2014
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