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. 1978 Mar;75(3):1433–1436. doi: 10.1073/pnas.75.3.1433

DNA cloning in Bacillus subtilis.

S D Ehrlich
PMCID: PMC411486  PMID: 418413

Abstract

A plasmid pC194, encoding resistance to chloramphenicol, can serve as a cloning vector in Bacillus subtilis 168 for other HindIII-cleaved DNA segments. Replicons constructed by linking pC194 to several Escherichia coli plasmids can be used to introduce and compare the expression of the same genes in these two bacterial hosts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borenstein S., Ephrati-Elizur E. Spontaneous release of DNA in sequential genetic order by Bacillus subtilis. J Mol Biol. 1969 Oct 14;45(1):137–152. doi: 10.1016/0022-2836(69)90216-2. [DOI] [PubMed] [Google Scholar]
  2. Chang A. C., Cohen S. N. Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1030–1034. doi: 10.1073/pnas.71.4.1030. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen S. N., Chang A. C., Boyer H. W., Helling R. B. Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240–3244. doi: 10.1073/pnas.70.11.3240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Graaff J., Elwell L. P., Falkow S. Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b. J Bacteriol. 1976 Apr;126(1):439–446. doi: 10.1128/jb.126.1.439-446.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ehrlich S. D., Bursztyn-Pettegrew H., Stroynowski I., Lederberg J. Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4145–4149. doi: 10.1073/pnas.73.11.4145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ehrlich S. D. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680–1682. doi: 10.1073/pnas.74.4.1680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harris-Warrick R. M., Elkana Y., Ehrlich S. D., Lederberg J. Electrophoretic separation of Bacillus subtilis genes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2207–2211. doi: 10.1073/pnas.72.6.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jacob A. E., Cresswell J. M., Hedges R. W., Coetzee J. N., Beringer J. E. Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4. Mol Gen Genet. 1976 Sep 23;147(3):315–323. doi: 10.1007/BF00582883. [DOI] [PubMed] [Google Scholar]
  10. Lovett P. S., Duvall E. J., Keggins K. M. Bacillus pumilus plasmid pPL10: properties and insertion into Bacillus subtilis 168 by transformation. J Bacteriol. 1976 Aug;127(2):817–828. doi: 10.1128/jb.127.2.817-828.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mahler I., Halvorson H. O. Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule. J Bacteriol. 1977 Jul;131(1):374–377. doi: 10.1128/jb.131.1.374-377.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Roberts M., Elwell L. P., Falkow S. Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae. J Bacteriol. 1977 Aug;131(2):557–563. doi: 10.1128/jb.131.2.557-563.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sgaramella V., Ehrlich S. D., Bursztyn H., Lederberg J. Enhancement of transfecting activity of bacteriophage P22 DNA upon exonucleolytic erosion. J Mol Biol. 1976 Aug 25;105(4):587–602. doi: 10.1016/0022-2836(76)90237-0. [DOI] [PubMed] [Google Scholar]
  14. Tanaka T., Kuroda M., Sakaguchi K. Isolation and characterization of four plasmids from Bacillus subtilis. J Bacteriol. 1977 Mar;129(3):1487–1494. doi: 10.1128/jb.129.3.1487-1494.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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