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. 1979 Nov;76(11):5465–5469. doi: 10.1073/pnas.76.11.5465

Distinctive nucleotide sequences of promoters recognized by RNA polymerase containing a phage-coded "sigma-like" protein.

C Talkington, J Pero
PMCID: PMC411669  PMID: 118447

Abstract

We report the nucleotide sequences of two promoters for bacteriophage SP01 "middle" genes. These promoters are recognized by a modified form of Bacillus subtilis RNA polymerase that contains a phage-coded "sigma-like" regulatory protein (gp28) in place of the bacterial sigma factor. Both promoters shared the identical hexanucleotide 5'A-G-G-A-G-A at about 35 base pairs preceding the start point of transcription and the identical heptanucleotide 5'-T-T-T-A-T-T-T (T is the thymine analog 5-hydroxymethyluracil in SP01 DNA) located about 10 base pairs preceding the transcriptional start point. The significance of these sequences in comparison with nucleotide sequences of promoters recognized by sigma-containing RNA polymerases is discussed.

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Selected References

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  1. Berkner K. L., Folk W. R. Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA. J Biol Chem. 1977 May 25;252(10):3176–3184. [PubMed] [Google Scholar]
  2. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duffy J. J., Geiduschek E. P. Purification of a positive regulatory subunit from phage SP01-modified RNA polymerase. Nature. 1977 Nov 3;270(5632):28–32. doi: 10.1038/270028a0. [DOI] [PubMed] [Google Scholar]
  4. Duffy J. J., Geiduschek E. P. RNA polymerase from phage SP01-infected and uninfected Bacillus subtilis. J Biol Chem. 1975 Jun 25;250(12):4530–4541. [PubMed] [Google Scholar]
  5. Fox T. D. Identification of phage SP01 proteins coded by regulatory genes 33 and 34. Nature. 1976 Aug 26;262(5571):748–753. doi: 10.1038/262748a0. [DOI] [PubMed] [Google Scholar]
  6. Fox T. D., Losick R., Pero J. Regulatory gene 28 of bacteriophage SPO1 codes for a phage-induced subunit of RNA polymerase. J Mol Biol. 1976 Mar 5;101(3):427–433. doi: 10.1016/0022-2836(76)90157-1. [DOI] [PubMed] [Google Scholar]
  7. Fujita D. J., Ohlsson-Wilhelm B. M., Geiduschek E. P. Transcription during bacteriophage SPO1 development: mutations affecting the program of viral transcription. J Mol Biol. 1971 Apr 28;57(2):301–317. doi: 10.1016/0022-2836(71)90348-2. [DOI] [PubMed] [Google Scholar]
  8. Galas D. J., Schmitz A. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Res. 1978 Sep;5(9):3157–3170. doi: 10.1093/nar/5.9.3157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hsieh T., Wang J. C. Physiochemical studies on interactions between DNA and RNA polymerase. Ultraviolet absorption measurements. Nucleic Acids Res. 1978 Sep;5(9):3337–3345. doi: 10.1093/nar/5.9.3337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnsrud L. Contacts between Escherichia coli RNA polymerase and a lac operon promoter. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5314–5318. doi: 10.1073/pnas.75.11.5314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mozola M. A., Friedman D. I., Crawford C. L., Wulff D. L., Shimatake H., Rosenberg M. Mutations reducing the activity of c17, a promoter of phage lambda formed by a tandem duplication. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1122–1125. doi: 10.1073/pnas.76.3.1122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. OKUBO S., STRAUSS B., STODOLSKY M. THE POSSIBLE ROLE OF RECOMBINATION IN THE INFECTION OF COMPETENT BACILLUS SUBTILIS BY BACTERIOPHAGE DEOXYRIBONUCLEIC ACID. Virology. 1964 Dec;24:552–562. doi: 10.1016/0042-6822(64)90207-7. [DOI] [PubMed] [Google Scholar]
  14. Pero J., Hannett N. M., Talkington C. Restriction cleavage map of SP01 DNA: general location of early, middle, and late genes. J Virol. 1979 Jul;31(1):156–171. doi: 10.1128/jvi.31.1.156-171.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pero J., Nelson J., Fox T. D. Highly asymmetric transcription by RNA polymerase containing phage-SP01-induced polypeptides and a new host protein. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1589–1593. doi: 10.1073/pnas.72.4.1589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ptashne M., Backman K., Humayun M. Z., Jeffrey A., Maurer R., Meyer B., Sauer R. T. Autoregulation and function of a repressor in bacteriophage lambda. Science. 1976 Oct 8;194(4261):156–161. doi: 10.1126/science.959843. [DOI] [PubMed] [Google Scholar]
  17. Schmitz A., Galas D. J. The interaction of RNA polymerase and lac repressor with the lac control region. Nucleic Acids Res. 1979 Jan;6(1):111–137. doi: 10.1093/nar/6.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Siebenlist U. Nucleotide sequence of the three major early promoters of bacteriophage T7. Nucleic Acids Res. 1979;6(5):1895–1907. doi: 10.1093/nar/6.5.1895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Siebenlist U. RNA polymerase unwinds an 11-base pair segment of a phage T7 promoter. Nature. 1979 Jun 14;279(5714):651–652. doi: 10.1038/279651a0. [DOI] [PubMed] [Google Scholar]
  20. Spiegelman G. B., Whiteley H. R. Bacteriophage SP82 induced modifications of Bacillus subtilis RNA polymerase result in the recognition of additional RNA synthesis initiation sites on phage DNA. Biochem Biophys Res Commun. 1978 Apr 14;81(3):1058–1065. doi: 10.1016/0006-291x(78)91458-4. [DOI] [PubMed] [Google Scholar]
  21. Talkington C., Pero J. Promoter recognition by phage SP01-modified RNA polymerase. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1185–1189. doi: 10.1073/pnas.75.3.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Talkington C., Pero J. Restriction fragment analysis of the temporal program of bacteriophage SPO1 transcription and its control by phage-modified RNA polymerases. Virology. 1977 Dec;83(2):365–379. doi: 10.1016/0042-6822(77)90181-7. [DOI] [PubMed] [Google Scholar]
  23. Tijan R., Pero J. Bacteriophage SP01 regulatory proteins directing late gene transcription in vitro. Nature. 1976 Aug 26;262(5571):753–757. doi: 10.1038/262753a0. [DOI] [PubMed] [Google Scholar]

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