Abstract
Photobleaching in a two-dimensional periodic pattern has been used to characterize the motion of succinylconcanavalin A receptors on the surfaces of mouse fibroblasts. These receptors were found to exhibit anisotropic two-dimensional diffusion in adherent cells having parallel stress fibers. Diffusion coefficients of the order of 10(-11) cm2/sec and 10(-12) cm2/sec were measured for diffusive motions parallel and perpendicular to these fibers. The determination of the anisotropic motion used a two-dimensional Fourier analysis. A study was made of the effect of changes in cell shape on the photobleach pattern.
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- Ash J. F., Louvard D., Singer S. J. Antibody-induced linkages of plasma membrane proteins to intracellular actomyosin-containing filaments in cultured fibroblasts. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5584–5588. doi: 10.1073/pnas.74.12.5584. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berlin R. D., Oliver J. M. Analogous ultrastructure and surface properties during capping and phagocytosis in leukocytes. J Cell Biol. 1978 Jun;77(3):789–804. doi: 10.1083/jcb.77.3.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bretscher M. S. Directed lipid flow in cell membranes. Nature. 1976 Mar 4;260(5546):21–23. doi: 10.1038/260021a0. [DOI] [PubMed] [Google Scholar]
- Dunham P. B., Goldstein I. M., Weissmann G. Potassium and amino acid transport in human leukocytes exposed to phagocytic stimuli. J Cell Biol. 1974 Oct;63(1):215–226. doi: 10.1083/jcb.63.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelman G. M. Surface modulation in cell recognition and cell growth. Science. 1976 Apr 16;192(4236):218–226. doi: 10.1126/science.769162. [DOI] [PubMed] [Google Scholar]
- Gunther G. R., Wang J. L., Yahara I., Cunningham B. A., Edelman G. M. Concanavalin A derivatives with altered biological activities. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1012–1016. doi: 10.1073/pnas.70.4.1012. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nicolson G. L. Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over surface components. Biochim Biophys Acta. 1976 Apr 13;457(1):57–108. doi: 10.1016/0304-4157(76)90014-9. [DOI] [PubMed] [Google Scholar]
- Poo M. M., Poo W. J., Lam J. W. Lateral electrophoresis and diffusion of Concanavalin A receptors in the membrane of embryonic muscle cell. J Cell Biol. 1978 Feb;76(2):483–501. doi: 10.1083/jcb.76.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubenstein J. L., Smith B. A., McConnell H. M. Lateral diffusion in binary mixtures of cholesterol and phosphatidylcholines. Proc Natl Acad Sci U S A. 1979 Jan;76(1):15–18. doi: 10.1073/pnas.76.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith B. A., McConnell H. M. Determination of molecular motion in membranes using periodic pattern photobleaching. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2759–2763. doi: 10.1073/pnas.75.6.2759. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith L. M., Smith B. A., McConnell H. M. Lateral diffusion of M-13 coat protein in model membranes. Biochemistry. 1979 May 29;18(11):2256–2259. doi: 10.1021/bi00578a019. [DOI] [PubMed] [Google Scholar]
- Smolen J. E., Weissmann G. Mg2+-ATPase as a membrane ecto-enzyme of human granulocytes. Inhibitors, activators and response to phagocytosis. Biochim Biophys Acta. 1978 Oct 4;512(3):525–538. doi: 10.1016/0005-2736(78)90162-1. [DOI] [PubMed] [Google Scholar]
- Tsan M. F., Berlin R. D. Effect of phagocytosis on membrane transport of nonelectrolytes. J Exp Med. 1971 Oct 1;134(4):1016–1035. doi: 10.1084/jem.134.4.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]