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. 1979 Dec;76(12):6481–6485. doi: 10.1073/pnas.76.12.6481

Proteases in cellular slime mold development: evidence for their involvement.

D Fong, J T Bonner
PMCID: PMC411889  PMID: 293735

Abstract

Protein degradation appears to be essential for normal differentiation in the cellular slime mold Dictyostelium discoideum. Several protease inhibitors block normal differentiation, and in most cases this inhibition can be reversed by addition of amino acids. For example, chloroquine, which inhibits slime mold cathepsin B activity, interferred with development by blocking sorocarp formation, and this inhibition was reversed by the addition of amino acids. Tosyllysyl chloromethyl ketone also blocked development, and this inhibition was reversed by simultaneous additions of amino acids and glutathione. Moreover, the addition of antipain and leupeptin delayed sorocarp formation. These results, together with the finding reported earlier that cathepsin B activity is differentially localized in the prestalk-prespore zones of the migrating slugs, suggest that proteolysis might play a regulatory role in cellular slime mold differentiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alton T. H., Lodish H. F. Developmental changes in messenger RNAs and protein synthesis in Dictyostelium discoideum. Dev Biol. 1977 Oct 1;60(1):180–206. doi: 10.1016/0012-1606(77)90118-x. [DOI] [PubMed] [Google Scholar]
  2. Bonner J. T., Barkley D. S., Hall E. M., Konijn T. M., Mason J. W., O'Keefe G., 3rd, Wolfe P. B. Acrasin, Acrasinase, and the sensitivity to acrasin in Dictyostelium discoideum. Dev Biol. 1969 Jul;20(1):72–87. doi: 10.1016/0012-1606(69)90005-0. [DOI] [PubMed] [Google Scholar]
  3. Chia W. K. Induction of stalk cell differentiation by cyclic-AMP in a susceptible variant of Dictyostelium discoideum. Dev Biol. 1975 Jun;44(2):239–252. doi: 10.1016/0012-1606(75)90395-4. [DOI] [PubMed] [Google Scholar]
  4. De Toma F. J., Kindwall K. E., Reardon C. A. The effect of tosyl lysine chloromethyl ketone on the activity of uridine diphosphoglucose pyrophosphorylase of the cellular slime mold Dictyostelium discoideum. Biochem Biophys Res Commun. 1977 Jan 24;74(2):350–355. doi: 10.1016/0006-291x(77)90311-4. [DOI] [PubMed] [Google Scholar]
  5. Farnsworth P. A., Loomis W. F. A barrier to diffusion in pseudoplasmodia of Dictyostelium discoideum. Dev Biol. 1974 Nov;41(1):77–83. doi: 10.1016/0012-1606(74)90284-x. [DOI] [PubMed] [Google Scholar]
  6. Fong D., Rutherford C. L. Protease activity during cell differentiation of the cellular slime mold Dictyostelium discoideum. J Bacteriol. 1978 May;134(2):521–527. doi: 10.1128/jb.134.2.521-527.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gustafson G. L., Thon L. A. Evidence for phosphoryl moieties in a proteinase from Dictyostelium discoideum. Biochem Biophys Res Commun. 1979 Feb 14;86(3):667–673. doi: 10.1016/0006-291x(79)91765-0. [DOI] [PubMed] [Google Scholar]
  8. Hames B. D., Ashworth J. M. The metabolism of macromolecules during the differentiation of Myxamoebae of the cellular slime mould Dictyostelium discoideum containing different amounts of glycogen. Biochem J. 1974 Aug;142(2):301–315. doi: 10.1042/bj1420301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kelleher J. K., Kelly P. J., Wright B. E. Amino acid catabolism and malic enzyme in differentiating Dictyostelium discoideum. J Bacteriol. 1979 May;138(2):467–474. doi: 10.1128/jb.138.2.467-474.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kempner E. S., Miller J. H. The molecular biology of Euglena gracilis. IX. Amino acid pool composition. J Protozool. 1974 May;21(2):363–367. doi: 10.1111/j.1550-7408.1974.tb03671.x. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Marin F. T. Regulation of development in Dictyostelium discoideum: I. Initiation of the growth to development transition by amino acid starvation. Dev Biol. 1976 Jan;48(1):110–117. doi: 10.1016/0012-1606(76)90050-6. [DOI] [PubMed] [Google Scholar]
  13. North M. J., Harwood J. M. Multiple acid proteinases in the cellular slime mould Dictyostelium discoideum. Biochim Biophys Acta. 1979 Jan 12;566(1):222–233. doi: 10.1016/0005-2744(79)90264-x. [DOI] [PubMed] [Google Scholar]
  14. North M. J. Inhibition of acid proteinase from Dictyostelium discoideum [proceedings]. Biochem Soc Trans. 1978;6(2):400–403. doi: 10.1042/bst0060400. [DOI] [PubMed] [Google Scholar]
  15. O'Day D. H. Acid protease activity during germination of microcysts of the cellular slime mold Polysphondylium pallidum. J Bacteriol. 1976 Jan;125(1):8–13. doi: 10.1128/jb.125.1.8-13.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ogino K., Nakashima K. Purification of rabbit liver cathepsin B1. J Biochem. 1974 Apr;75(4):723–730. doi: 10.1093/oxfordjournals.jbchem.a130445. [DOI] [PubMed] [Google Scholar]
  17. Ohkuma S., Poole B. Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3327–3331. doi: 10.1073/pnas.75.7.3327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Penn A., Lake S., Timourian H., Gledhill B. L. Division delay in sea urchin embryos induced by a specific protease inhibitor. Exp Cell Res. 1976 Jan;97:164–174. doi: 10.1016/0014-4827(76)90665-0. [DOI] [PubMed] [Google Scholar]
  19. Rossomando E. F., Maldonado B., Crean E. V., Kollar E. J. Protease secretion during onset of development in Dictyostelium discoideum. J Cell Sci. 1978 Apr;30:305–318. doi: 10.1242/jcs.30.1.305. [DOI] [PubMed] [Google Scholar]
  20. Smith R., Turk V. Cathepsin D: rapid isolation by affinity chromatography on haemoglobin-agarose resin. Eur J Biochem. 1974 Oct 1;48(1):245–254. doi: 10.1111/j.1432-1033.1974.tb03762.x. [DOI] [PubMed] [Google Scholar]
  21. WHITE G. J., SUSSMAN M. Metabolism of major cell components during slime mold morphogenesis. Biochim Biophys Acta. 1961 Oct 28;53:285–293. doi: 10.1016/0006-3002(61)90441-3. [DOI] [PubMed] [Google Scholar]
  22. WRIGHT B. E., ANDERSON M. L. Protein and amino acid turnover during differentiation in the slime mold. I. Utilization of endogenous amino acids and proteins. Biochim Biophys Acta. 1960 Sep 9;43:62–66. doi: 10.1016/0006-3002(60)90407-8. [DOI] [PubMed] [Google Scholar]
  23. Watts D. J., Ashworth J. M. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970 Sep;119(2):171–174. doi: 10.1042/bj1190171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wiener E., Ashworth J. M. The isolation and characterization of lysosomal particles from myxamoebae of the cellular slime mould Dictyostelium discoideum. Biochem J. 1970 Jul;118(3):505–512. doi: 10.1042/bj1180505. [DOI] [PMC free article] [PubMed] [Google Scholar]

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