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. 1995 Aug 15;92(17):8011–8015. doi: 10.1073/pnas.92.17.8011

An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.

M A Stamnes 1, M W Craighead 1, M H Hoe 1, N Lampen 1, S Geromanos 1, P Tempst 1, J E Rothman 1
PMCID: PMC41276  PMID: 7644530

Abstract

We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad repeats found in coiled coils. When the yeast p24 isoform, yp24A, is knocked out in a strain defective for vesicle fusion, a dramatic reduction in the accumulation of transport vesicles is observed. Together, these results indicate a role for this protein family in the budding of coatamer-coated and other species of coated vesicles.

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Selected References

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