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. 1979 Oct;76(10):4927–4931. doi: 10.1073/pnas.76.10.4927

Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.

R P Ricciardi, J S Miller, B E Roberts
PMCID: PMC413051  PMID: 291909

Abstract

We describe a simple procedure for isolating specific mRNAs and for mapping them to the regions of the DNA from which they originate. The method involves hybridization of total cytoplasmic RNA to restriction fragments of DNA that have been fractionated in agarose gels and immobilized on nitrocellulose filters. The hybridization-selected RNAs are eluted and translated in a cell-free system in order to identify their encoded polypeptides. Optimal hybridization and filter washing conditions are given for selection of mRNAs from adenovirus 2-infected cells and transformed cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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