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. 1993 May;12(5):2039–2048. doi: 10.1002/j.1460-2075.1993.tb05853.x

The Aspergillus nidulans yA gene is regulated by abaA.

R Aramayo 1, W E Timberlake 1
PMCID: PMC413426  PMID: 8491194

Abstract

The developmentally regulated Aspergillus nidulans yA gene encodes a p-diphenol oxidase that is needed for synthesis of green conidial pigment. We subjected the yA 5' flanking region to mutational analysis in A. nidulans and Saccharomyces cerevisiae to identify DNA sequence elements involved in its transcriptional control, and identified two functionally distinct elements. Element I contained potential BrlA binding sites and was required for full level yA transcription, but not for developmental regulation in the presence of element II. Element II contained putative TEF-1 binding sites flanking a CCAAT element and was sufficient for developmental regulation of transcription. Mutation of the TEF-1 binding sites eliminated developmental regulation, whereas mutation of the CCAAT element led to elevated levels of transcription. Element II was also sufficient to induce transcription in S. cerevisiae when the A.nidulans developmental regulatory gene abaA was expressed from the GAL1 promoter. As AbaA and TEF-1 possess similar DNA binding domains, the abaA-yA interaction in yeast is probably direct. Thus, abaA appears to be a direct activator of yA, but yA regulation may also involve interactions with BrlA and a member of the CCAAT class of DNA binding proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams T. H., Boylan M. T., Timberlake W. E. brlA is necessary and sufficient to direct conidiophore development in Aspergillus nidulans. Cell. 1988 Jul 29;54(3):353–362. doi: 10.1016/0092-8674(88)90198-5. [DOI] [PubMed] [Google Scholar]
  2. Adams T. H., Timberlake W. E. Upstream elements repress premature expression of an Aspergillus developmental regulatory gene. Mol Cell Biol. 1990 Sep;10(9):4912–4919. doi: 10.1128/mcb.10.9.4912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Andrianopoulos A., Timberlake W. E. ATTS, a new and conserved DNA binding domain. Plant Cell. 1991 Aug;3(8):747–748. doi: 10.1105/tpc.3.8.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Aramayo R., Timberlake W. E. Sequence and molecular structure of the Aspergillus nidulans yA (laccase I) gene. Nucleic Acids Res. 1990 Jun 11;18(11):3415–3415. doi: 10.1093/nar/18.11.3415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boylan M. T., Mirabito P. M., Willett C. E., Zimmerman C. R., Timberlake W. E. Isolation and physical characterization of three essential conidiation genes from Aspergillus nidulans. Mol Cell Biol. 1987 Sep;7(9):3113–3118. doi: 10.1128/mcb.7.9.3113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  7. Bürglin T. R. The TEA domain: a novel, highly conserved DNA-binding motif. Cell. 1991 Jul 12;66(1):11–12. doi: 10.1016/0092-8674(91)90132-i. [DOI] [PubMed] [Google Scholar]
  8. Campbell S., Inamdar M., Rodrigues V., Raghavan V., Palazzolo M., Chovnick A. The scalloped gene encodes a novel, evolutionarily conserved transcription factor required for sensory organ differentiation in Drosophila. Genes Dev. 1992 Mar;6(3):367–379. doi: 10.1101/gad.6.3.367. [DOI] [PubMed] [Google Scholar]
  9. Casadaban M. J., Martinez-Arias A., Shapira S. K., Chou J. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast. Methods Enzymol. 1983;100:293–308. doi: 10.1016/0076-6879(83)00063-4. [DOI] [PubMed] [Google Scholar]
  10. Chang Y. C., Timberlake W. E. Identification of Aspergillus brlA response elements (BREs) by genetic selection in yeast. Genetics. 1993 Jan;133(1):29–38. doi: 10.1093/genetics/133.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Clutterbuck A. J. A mutational analysis of conidial development in Aspergillus nidulans. Genetics. 1969 Oct;63(2):317–327. doi: 10.1093/genetics/63.2.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Clutterbuck A. J. Absence of laccase from yellow-spored mutants of Aspergillus nidulans. J Gen Microbiol. 1972 May;70(3):423–435. doi: 10.1099/00221287-70-3-423. [DOI] [PubMed] [Google Scholar]
  13. Company M., Errede B. A Ty1 cell-type-specific regulatory sequence is a recognition element for a constitutive binding factor. Mol Cell Biol. 1988 Dec;8(12):5299–5309. doi: 10.1128/mcb.8.12.5299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Davidson I., Xiao J. H., Rosales R., Staub A., Chambon P. The HeLa cell protein TEF-1 binds specifically and cooperatively to two SV40 enhancer motifs of unrelated sequence. Cell. 1988 Sep 23;54(7):931–942. doi: 10.1016/0092-8674(88)90108-0. [DOI] [PubMed] [Google Scholar]
  15. Davis M. A., Cobbett C. S., Hynes M. J. An amdS-lacZ fusion for studying gene regulation in Aspergillus. Gene. 1988 Mar 31;63(2):199–212. doi: 10.1016/0378-1119(88)90525-2. [DOI] [PubMed] [Google Scholar]
  16. Errede B., Company M., Hutchison C. A., 3rd Ty1 sequence with enhancer and mating-type-dependent regulatory activities. Mol Cell Biol. 1987 Jan;7(1):258–265. doi: 10.1128/mcb.7.1.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Fantes P. A., Roberts C. F. Beta-galactosidase activity and lactose utilization in Aspergillus nidulans. J Gen Microbiol. 1973 Aug;77(2):417–486. doi: 10.1099/00221287-77-2-417. [DOI] [PubMed] [Google Scholar]
  18. Fromental C., Kanno M., Nomiyama H., Chambon P. Cooperativity and hierarchical levels of functional organization in the SV40 enhancer. Cell. 1988 Sep 23;54(7):943–953. doi: 10.1016/0092-8674(88)90109-2. [DOI] [PubMed] [Google Scholar]
  19. Guarente L. Regulatory proteins in yeast. Annu Rev Genet. 1987;21:425–452. doi: 10.1146/annurev.ge.21.120187.002233. [DOI] [PubMed] [Google Scholar]
  20. Gómez-Pardo E., Peñalva M. A. The upstream region of the IPNS gene determines expression during secondary metabolism in Aspergillus nidulans. Gene. 1990 Apr 30;89(1):109–115. doi: 10.1016/0378-1119(90)90212-a. [DOI] [PubMed] [Google Scholar]
  21. Hamer J. E., Timberlake W. E. Functional organization of the Aspergillus nidulans trpC promoter. Mol Cell Biol. 1987 Jul;7(7):2352–2359. doi: 10.1128/mcb.7.7.2352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  23. Henikoff S. Unidirectional digestion with exonuclease III in DNA sequence analysis. Methods Enzymol. 1987;155:156–165. doi: 10.1016/0076-6879(87)55014-5. [DOI] [PubMed] [Google Scholar]
  24. Kunkel T. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A. 1985 Jan;82(2):488–492. doi: 10.1073/pnas.82.2.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Käfer E. Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet. 1977;19:33–131. doi: 10.1016/s0065-2660(08)60245-x. [DOI] [PubMed] [Google Scholar]
  26. Laloux I., Dubois E., Dewerchin M., Jacobs E. TEC1, a gene involved in the activation of Ty1 and Ty1-mediated gene expression in Saccharomyces cerevisiae: cloning and molecular analysis. Mol Cell Biol. 1990 Jul;10(7):3541–3550. doi: 10.1128/mcb.10.7.3541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Law D. J., Timberlake W. E. Developmental regulation of laccase levels in Aspergillus nidulans. J Bacteriol. 1980 Nov;144(2):509–517. doi: 10.1128/jb.144.2.509-517.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Lohr D. Organization of the GAL1-GAL10 intergenic control region chromatin. Nucleic Acids Res. 1984 Nov 26;12(22):8457–8474. doi: 10.1093/nar/12.22.8457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Mar J. H., Ordahl C. P. A conserved CATTCCT motif is required for skeletal muscle-specific activity of the cardiac troponin T gene promoter. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6404–6408. doi: 10.1073/pnas.85.17.6404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Mar J. H., Ordahl C. P. M-CAT binding factor, a novel trans-acting factor governing muscle-specific transcription. Mol Cell Biol. 1990 Aug;10(8):4271–4283. doi: 10.1128/mcb.10.8.4271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Marsh J. L., Erfle M., Wykes E. J. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene. 1984 Dec;32(3):481–485. doi: 10.1016/0378-1119(84)90022-2. [DOI] [PubMed] [Google Scholar]
  32. Marshall M. A., Timberlake W. E. Aspergillus nidulans wetA activates spore-specific gene expression. Mol Cell Biol. 1991 Jan;11(1):55–62. doi: 10.1128/mcb.11.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Mayorga M. E., Timberlake W. E. Isolation and molecular characterization of the Aspergillus nidulans wA gene. Genetics. 1990 Sep;126(1):73–79. doi: 10.1093/genetics/126.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Mirabito P. M., Adams T. H., Timberlake W. E. Interactions of three sequentially expressed genes control temporal and spatial specificity in Aspergillus development. Cell. 1989 Jun 2;57(5):859–868. doi: 10.1016/0092-8674(89)90800-3. [DOI] [PubMed] [Google Scholar]
  35. O'Hara E. B., Timberlake W. E. Molecular characterization of the Aspergillus nidulans yA locus. Genetics. 1989 Feb;121(2):249–254. doi: 10.1093/genetics/121.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. PONTECORVO G., ROPER J. A., HEMMONS L. M., MACDONALD K. D., BUFTON A. W. J. The genetics of Aspergillus nidulans. Adv Genet. 1953;5:141–238. doi: 10.1016/s0065-2660(08)60408-3. [DOI] [PubMed] [Google Scholar]
  37. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  38. Sewall T. C., Mims C. W., Timberlake W. E. abaA controls phialide differentiation in Aspergillus nidulans. Plant Cell. 1990 Aug;2(8):731–739. doi: 10.1105/tpc.2.8.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Sikorski R. S., Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 1989 May;122(1):19–27. doi: 10.1093/genetics/122.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Stringer M. A., Dean R. A., Sewall T. C., Timberlake W. E. Rodletless, a new Aspergillus developmental mutant induced by directed gene inactivation. Genes Dev. 1991 Jul;5(7):1161–1171. doi: 10.1101/gad.5.7.1161. [DOI] [PubMed] [Google Scholar]
  41. Timberlake W. E., Marshall M. A. Genetic engineering of filamentous fungi. Science. 1989 Jun 16;244(4910):1313–1317. doi: 10.1126/science.2525275. [DOI] [PubMed] [Google Scholar]
  42. Timberlake W. E., Marshall M. A. Genetic regulation of development in Aspergillus nidulans. Trends Genet. 1988 Jun;4(6):162–169. doi: 10.1016/0168-9525(88)90022-4. [DOI] [PubMed] [Google Scholar]
  43. Timberlake W. E. Molecular genetics of Aspergillus development. Annu Rev Genet. 1990;24:5–36. doi: 10.1146/annurev.ge.24.120190.000253. [DOI] [PubMed] [Google Scholar]
  44. Timberlake W. E. Temporal and spatial controls of Aspergillus development. Curr Opin Genet Dev. 1991 Oct;1(3):351–357. doi: 10.1016/s0959-437x(05)80299-0. [DOI] [PubMed] [Google Scholar]
  45. West R. W., Jr, Yocum R. R., Ptashne M. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG. Mol Cell Biol. 1984 Nov;4(11):2467–2478. doi: 10.1128/mcb.4.11.2467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Xiao J. H., Davidson I., Matthes H., Garnier J. M., Chambon P. Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1. Cell. 1991 May 17;65(4):551–568. doi: 10.1016/0092-8674(91)90088-g. [DOI] [PubMed] [Google Scholar]
  47. Yelton M. M., Hamer J. E., Timberlake W. E. Transformation of Aspergillus nidulans by using a trpC plasmid. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1470–1474. doi: 10.1073/pnas.81.5.1470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. van Gorcom R. F., Pouwels P. H., Goosen T., Visser J., van den Broek H. W., Hamer J. E., Timberlake W. E., van den Hondel C. A. Expression of an Escherichia coli beta-galactosidase fusion gene in Aspergillus nidulans. Gene. 1985;40(1):99–106. doi: 10.1016/0378-1119(85)90028-9. [DOI] [PubMed] [Google Scholar]

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