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. 1978 Jan;19(1):51–57. doi: 10.1128/iai.19.1.51-57.1978

Alteration of clearance function by group A streptococcal pyrogenic exotoxin and its relation to suppression of the antibody response.

C M Cunningham, D W Watson
PMCID: PMC414047  PMID: 342415

Abstract

The effect of purified group A streptococcal pyrogenic exotoxin (SPE) type A on the processing of and antibody response to sheep erythrocytes (SRBC) was studied in BALB/cWat mice. The rate of clearance of 51Cr-labeled SRBC from the bloodstream was decreased 3 or 24 h following a single intravenous injection of 1 or 10 microgram of SPE. Delayed uptake of label was observed in both the livers and spleens of SPE-treated mice, suggesting an inhibitory effect of the toxin on phagocytic cells of the reticuloendothelial system. Three daily intravenous injections of 0.1 or 1 microgram of purified SPE type A suppressed the early immunoglobulin response to SRBC. The role of altered macrophage function in producing the immunosuppression was tested in macrophage transfer experiments. SPE treatment suppressed the antibody response to SRBC transferred by normal macrophages, indicating that the immunosuppressive effect of the toxin was not due solely to altered antigen processing by macrophages.

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Selected References

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  1. Cruchaud A., Unanue E. R. Fate and immunogenicity of antigens endocytosed by macrophages: a study using foreign red cells and immunoglobulin G. J Immunol. 1971 Nov;107(5):1329–1340. [PubMed] [Google Scholar]
  2. Cunningham C. M., Barsumian E. L., Watson D. W. Further purification of group A streptococcal pyrogenic exotoxin and characterization of the purified toxin. Infect Immun. 1976 Sep;14(3):767–775. doi: 10.1128/iai.14.3.767-775.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dutton R. W. Inhibitory and stimulatory effects of concanavalin A on the response of mouse spleen cell suspensions to antigen. I. Characterization of the inhibitory cell activity. J Exp Med. 1972 Dec 1;136(6):1445–1460. doi: 10.1084/jem.136.6.1445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Egan H. S., Reeder W. J., Ekstedt R. D. Effect of concanavalin A in vivo in suppressing the antibody response in mice. J Immunol. 1974 Jan;112(1):63–69. [PubMed] [Google Scholar]
  5. HALPERN B. N., BIOZZI G., BENACERRAF B., STIFFEL C. Phagocytosis of foreign red blood cells by the reticulo-endothelial system. Am J Physiol. 1957 Jun;189(3):520–526. doi: 10.1152/ajplegacy.1957.189.3.520. [DOI] [PubMed] [Google Scholar]
  6. HANNA E. E., WATSON D. W. HOST-PARASITE RELATIONSHIPS AMONG GROUP A STREPTOCOCCI. 3. DEPRESSION OF RETICULOENDOTHELIAL FUNCTION BY STREPTOCOCCAL PYROGENIC EXOTOXINS. J Bacteriol. 1965 Jan;89:154–158. doi: 10.1128/jb.89.1.154-158.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hale M. L., Hanna E. E. Deregulation of mouse antibody-forming cells by streptococcal pyrogenic exotoxin. II. Modification of spleen T-cell-complemented nude mouse PFC responses. Cell Immunol. 1976 Oct;26(2):168–177. doi: 10.1016/0008-8749(76)90361-0. [DOI] [PubMed] [Google Scholar]
  8. Hanna E. E., Hale M. Deregulation of mouse antibody-forming cells in vivo in cell culture by streptococcal pyrogenic exotoxin. Infect Immun. 1975 Feb;11(2):265–272. doi: 10.1128/iai.11.2.265-272.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanna E. E., Watson D. W. Enhanced immune response after immunosuppression by Streptococcal pyrogenic exotoxin. Infect Immun. 1973 Jun;7(6):1009–1011. doi: 10.1128/iai.7.6.1009-1011.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hanna E. E., Watson D. W. Host-parasite relationships among group A streptococci. IV. Suppression of antibody response by streptococcal pyrogenic exotoxin. J Bacteriol. 1968 Jan;95(1):14–21. doi: 10.1128/jb.95.1.14-21.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hríbalová V., Pospísil M. Lymphocyte-stimulating activity of scarlet fever toxin. Experientia. 1973 Jun 15;29(6):704–705. doi: 10.1007/BF01944787. [DOI] [PubMed] [Google Scholar]
  12. Kim Y. B., Watson D. W. A purified group A streptococcal pyrogenic exotoxin. Physiochemical and biological properties including the enhancement of susceptibility to endotoxin lethal shock. J Exp Med. 1970 Mar 1;131(3):611–622. doi: 10.1084/jem.131.3.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McClain D. A., Edelman G. M. Analysis of the stimulation-inhibition paradox exhibited by lymphocytes exposed to concanavalin A. J Exp Med. 1976 Dec 1;144(6):1494–1508. doi: 10.1084/jem.144.6.1494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Munson A. E., Regelson W., Wooles W. R. Tissue localization studies in evaluating the functional role of the RES. J Reticuloendothel Soc. 1970 Mar;7(3):366–374. [PubMed] [Google Scholar]
  16. Rich R. R., Pierce C. W. Biological expressions of lymphocyte activation. II. Generation of a population of thymus-derived suppressor lymphocytes. J Exp Med. 1973 Mar 1;137(3):649–659. doi: 10.1084/jem.137.3.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sabet T., Newlin C., Friedman H. The effect of RES blockade on cellular antibody formation to sheep erythrocytes. Proc Soc Exp Biol Med. 1968 May;128(1):274–278. doi: 10.3181/00379727-128-32996. [DOI] [PubMed] [Google Scholar]
  18. Schlievert P. M., Bettin K. M., Watson D. W. Purification and characterization of group A streptococcal pyrogenic exotoxin type C. Infect Immun. 1977 May;16(2):673–679. doi: 10.1128/iai.16.2.673-679.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Unanue E. R. The regulatory role of macrophages in antigenic stimulation. Adv Immunol. 1972;15:95–165. doi: 10.1016/s0065-2776(08)60684-7. [DOI] [PubMed] [Google Scholar]
  20. WATSON D. W. Host-parasite factors in group A streptococcal infections. Pyrogenic and other effects of immunologic distinct exotoxins related to scarlet fever toxins. J Exp Med. 1960 Feb 1;111:255–284. doi: 10.1084/jem.111.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WOOLES W. R., DILUZIO N. R. RETICULOENDOTHELIAL FUNCTION AND THE IMMUNE RESPONSE. Science. 1963 Nov 22;142(3595):1078–1080. doi: 10.1126/science.142.3595.1078. [DOI] [PubMed] [Google Scholar]
  22. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

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