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. 1978 Jan;19(1):160–169. doi: 10.1128/iai.19.1.160-169.1978

Antigens of Streptococcus mutans: Isolation of a Serotype-Specific and a Cross-Reactive Antigen from Walls of Strain V-100 (Serotype e) †

J R Wetherell Jr 1, A S Bleiweis 1
PMCID: PMC414062  PMID: 75187

Abstract

Two cell wall-associated polysaccharide antigens were extracted from purified cell walls of Streptococcus mutans serotype e strain V-100. One of these purified antigens (I) is specific for serotype e, whereas the other (II) has antigenic determinants reactive with both heterologous anti-serotype c serum (GS-5) and the homologous (e) serum. When crude formamide extracts of V-100 cell walls were loaded onto a Cellex-D column and eluted with a linear gradient of ammonium carbonate (0.02 to 0.40 M), the two products mentioned above could be recovered. The purified, antigenically reactive products (I and II) were each composed only of rhamnose and glucose in approximately a 2:1 molar ratio. Immunoelectrophoresis of the crude formamide extract, peak I, and peak II showed the purified fractions to have opposite mobilities and the crude extract to have a mobility that encompassed both purified peaks when reacted with homologous antiserum (V-100). When these three fractions were immunoelectrophoresed and reacted with heterologous anti-serotype c serum (GS-5), only the anodic portion of the crude V-100 formamide extract and purified peak II formed precipitates. Ouchterlony analysis with homologous antiserum produced precipitin patterns between the crude formamide extract and both purified peaks, indicating complete identity. However, only crude extracts of V-100 and the purified peak II material reacted with heterologous (c) antiserum; peak I did not cross-react in these Ouchterlony assays. Hapten inhibition studies revealed that a β-glucosyl moiety is the immunodeterminant for serotype e and is present on each purified fraction. The basis of the cross-reaction between anti-c sera and the purified antigen II of e is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed] [Google Scholar]
  2. Bratthall D. Immunodiffusion studies on the serological specificity of streptococci resembling Streptococcus mutans. Odontol Revy. 1969;20(3):231–243. [PubMed] [Google Scholar]
  3. Bratthall D. Immunofluorescent identification of Streptococcus mutans. Odontol Revy. 1972;23(2):181–196. [PubMed] [Google Scholar]
  4. Edwardsson S. Characteristics of caries-inducing human streptococci resembling Streptococcus mutans. Arch Oral Biol. 1968 Jun;13(6):637–646. doi: 10.1016/0003-9969(68)90142-8. [DOI] [PubMed] [Google Scholar]
  5. Guggenheim B. Streptococci of dental plaques. Caries Res. 1968;2(2):147–163. doi: 10.1159/000259553. [DOI] [PubMed] [Google Scholar]
  6. Hamada S., Gill K., Slade H. D. Chemical and immunological properties of the type f polysaccharide antigen of Streptococcus mutans. Infect Immun. 1976 Jul;14(1):203–211. doi: 10.1128/iai.14.1.203-211.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hamada S., Slade H. D. Purification and immunochemical characterization of type e polysaccharide antigen of Streptococcus mutans. Infect Immun. 1976 Jul;14(1):68–76. doi: 10.1128/iai.14.1.68-76.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iacono V. J., Taubman M. A., Smith D. J., Levine M. J. Isolation and immunochemical characterization of the group-specific antigen of Streptococcus mutants 6715. Infect Immun. 1975 Jan;11(1):117–128. doi: 10.1128/iai.11.1.117-128.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Linzer R., Slade H. D. Purification and characterization of Streptococcus mutans group d cell wall polysaccharide antigen. Infect Immun. 1974 Aug;10(2):361–368. doi: 10.1128/iai.10.2.361-368.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mukasa H., Slade H. D. Extraction, purification, and chemical and immunological properties of the Streptococcus mutans group "a" polysaccharide cell wall antigen. Infect Immun. 1973 Aug;8(2):190–198. doi: 10.1128/iai.8.2.190-198.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mukasa H., Slade H. D. Structure and immunological specificity of the Streptococcus mutans group b cell wall antigen. Infect Immun. 1973 Apr;7(4):578–585. doi: 10.1128/iai.7.4.578-585.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Perch B., Kjems E., Ravn T. Biochemical and serological properties of Streptococcus mutans from various human and animal sources. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):357–370. doi: 10.1111/j.1699-0463.1974.tb02338.x. [DOI] [PubMed] [Google Scholar]
  14. Soprey P., Slade H. D. Chemical structure and immunological specificity of the streptococcal group e cell wall polysaccharide antigen. Infect Immun. 1971 May;3(5):653–658. doi: 10.1128/iai.3.5.653-658.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Van de Rijn I., Bleiweis A. S. Antigens of Streptococcus mutans. I. Characterization of a serotype-specific determinant from Streptococcus mutans. Infect Immun. 1973 May;7(5):795–804. doi: 10.1128/iai.7.5.795-804.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vaught R. M., Bleiweis A. S. Antigens of Streptococcus mutans. II. Characterization of an antigen resembling a glycerol teichoic acid in walls of strain BHT. Infect Immun. 1974 Jan;9(1):60–67. doi: 10.1128/iai.9.1.60-67.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wetherell J. R., Jr, Bleiweis A. S. Antigens of Streptococcus mutans: characterization of a polysaccharide antigen from walls of strain GS-5. Infect Immun. 1975 Dec;12(6):1341–1348. doi: 10.1128/iai.12.6.1341-1348.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ZINNER D. D., JABLON J. M., ARAN A. P., SASLAW M. S. EXPERIMENTAL CARIES INDUCED IN ANIMALS BY STREPTOCOCCI OF HUMAN ORIGIN. Proc Soc Exp Biol Med. 1965 Mar;118:766–770. doi: 10.3181/00379727-118-29964. [DOI] [PubMed] [Google Scholar]
  19. de Stoppelaar J. D., König K. G., Plasschaert A. J., van der Hoeven J. S. Decreased cariogenicity of a mutant of Streptococcus mutans. Arch Oral Biol. 1971 Aug;16(8):971–975. doi: 10.1016/0003-9969(71)90186-5. [DOI] [PubMed] [Google Scholar]

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